Thermo Fisher LCQ Fleet ion trap liquid chromatography-mass spectrometer

Thermo Fisher LCQ Fleet ion trap liquid chromatography-mass spectrometer

Introduction

Thermo Scientific ™ LCQ Fleet ™ The ion trap mass spectrometer is renowned for its high sensitivity, multi-stage mass spectrometry capability, and high stability and reliability, making it suitable for high-throughput analysis of complex samples. Equipped with automatic gain control (AGC), dynamic exclusion technology, and broadband excitation, ensuring high-quality acquisition of structural information. In terms of hardware, the Ion Max-S API ion source improves sensitivity and stability, and simplifies maintenance processes. The scanning function is diverse, covering multi-stage mass spectrometry scanning, MS/MS, SRM, etc., supporting comprehensive data acquisition and analysis. LCQ Fleet is widely used in unknown screening, natural compound identification, and complex proteomic research, providing reliable structural characterization and compound identification capabilities.

Thermo ScientificTM LCQ FleetTM inherits the sensitive multi-stage mass spectrometry capability, high stability, and reliability of the LCQTM series ion trap. This economical and practical ion trap mass spectrometer can perform high-throughput routine analysis on complex samples, providing high-quality structural information.

Technology:

-The acquired automatic gain control (AGC) ensures that the ion trap is filled with excellent ion quantities in any scanning mode

-Dynamic ExclusionTM technology ensures that all types of ions with low ion strength also have the opportunity for secondary and multi-stage mass spectrometry data collection

-WideBand Activation Technology can generate more structural information data

-Normalized collision energy technology NCETM eliminates mass discrimination effects in secondary mass spectrometry experiments of ion trap mass spectrometers, making the data generated by different instruments reproducible

-Pulse Q-splitting technology PQDTM ensures the ability to capture low-quality end ions

Hardware features: Ion Max-S API ion source

-Better sensitivity and stability

-Blow off gas technology reduces chemical noise

-Tilted spray angle interchangeable ion probe

-Detachable metal transfer capillary tube for maintenance of vacuum without disassembly

Scanning ability: multi-stage mass spectrometry scanning, MSn full scan: can perform sensitive full scan analysis, quickly screen unknown substances

-Select ion monitoring SIM to monitor the selected ions for analyzing the target compound

-MS/MS secondary mass spectrometry full scan can generate full scan spectra of sub ions, with higher sensitivity than any other ion trap mass spectrometer

-Selecting Reaction Monitoring (SRM) scanning for traditional LC/MS/MS quantitative analysis experiments

-Multi level mass spectrometry scanning enables multi-level MS experiments to study ion structure characteristics

-ZoomScanTM scanning has high resolution and full mass range scanning function, which can distinguish the isotopic distribution of ions. This function is usually used for the determination of charge valence states of peptides and organic metal ions

-TurboScanTM scanning is an ultra fast scanning method that can improve signal-to-noise ratio and acquisition rate

Main applications:

1. Reliable screening for general unknown substances

The intelligent data dependent collection feature of LCQ Fleet can quickly screen compounds to obtain the maximum amount of structural information. LCQ Fleet has the characteristics of short analysis cycle and high sensitivity, which can obtain sufficient information in one sample analysis to confirm unknown substances. Each fragmentation spectrum can be automatically retrieved from the spectral library and the retention time can be confirmed, and a single page report will be generated for each identified compound.

2. Identification of natural compounds

LCQ Fleet can greatly reduce the lengthy pre purification process while ensuring high-quality analysis results. This is particularly suitable for routine identification of complex natural products. Its advantages are as follows:

-The ability to quickly analyze complex samples reduces or avoids time-consuming pre purification steps

-The rapid analysis capability of LCQ Fleet is matched with the separation phase of Ulimate 3000 ultra-high speed liquid chromatography, which can quickly complete the analysis of a large number of samples

-The multi-level mass spectrometry analysis function ensures the reliability of structural characterization and the accuracy of compound identification

-The Ion MaxTM ion source with * functionality can meet various demanding analytical needs

-Various ionization methods, including ESI, APCI, APPI, etc., provide multiple flexible choices for obtaining sample information

-MassFrontierTM software can directly analyze data for compound identification and generate reports

3. Complex proteomics research

-Fast scanning technology solves the analysis problem of peptide co efflux in complex protein hydrolysates in real-time online analysis

-High sensitivity, capable of detecting trace peptide segments in complex mixtures

-High quality multilevel mass spectrometry makes peptide identification more reliable

-Pulse Q-dissociation (PQD) can detect low-quality fragments in iTRAQTM quantitative analysis

-Rapid enhanced resolution scanning and region scanning can determine the charge valence state of peptide ions

-High resolution separation (HRI) enables multi-stage mass spectrometry analysis to be free from interference from isotopic ions in proteomics analysis