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Shenzhen Amperex Biotechnology Co., Ltd
Target protein palmitoyl immunoblot detection kit (including HRP)
NegotiableUpdate on 05/06
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Overview
The target protein palmitoyl immunoblot detection kit (containing HRP) is used for palmitoyl site detection by immunoblotting after immune purification of the target protein and treatment with this kit.
Product Details
| Item No. | AM10314 |
| Product Name | IP-ABE Palmitoylation Kit For WB（含HRP）Target protein palmitoyl immunoblot detection kit (including HRP) |
| Product advantages: | Target protein palmitoyl immunoblot detection kit (including HRP)AM10314 was developed for the detection of specific protein palmitoyl modification sites in most studies. After immune purification of the target protein and treatment with this kit, palmitoyl sites were detected using immunoblotting. |
| Product Features: | The detection process is relatively fast and convenient, and can be combined with StrePDAvin HRP to detect the target protein modification site. The experimental operation is easy to operate, error free, and the results can be repeated after repeated testing |
| Operation steps: | Closed restoration capture detection |
| Reagent name_20rnxs: | LB Buffer 1,Blocking Reagent ,LB Buffer 2,HAM Reaction Buffer ,LB Buffer 3,Biotin Reaction Buffer,Streptavidin HRP |
| Reagent No. 20rnxs: | Solution I,Solution II,Solution III,Solution IV,Solution V ,SolutionVI,Solution VII |
| Volume_Form_20rnxs: | 50mL,500μL,50mL,1.2mL,50mL,15μL,5μL |
| Storage temperature_20rnxs: | 4℃,-20℃,4℃,-20℃,4℃,-20℃,-20℃ |
| Reagent name_4rnxs: | LB Buffer 1,Blocking Reagent ,LB Buffer 2,HAM Reaction Buffer ,LB Buffer 3,Biotin Reagent Buffer,Streptavidin HRP |
| Reagent ID: 4rnxs | Solution I,Solution II,Solution III,Solution IV,Solution V ,SolutionVI,Solution VII |
| Volume_Form_4rnxs: | 8.2mL,50μL,8.2mL,220μL,8.2mL,3μL,20μL |
| Storage temperature: 4rnxs | 4℃,-20℃,4℃,-20℃,4℃,-20℃,-20℃ |
Kit composition:
| Reagent name (20rnxs) | Reagent number | Volume (formal) | Storage temperature |
| LB Buffer 1 | Solution I | 50mL | 4℃ |
| Blocking Reagent | Solution II | 500μL | -20℃ |
| LB Buffer 2 | Solution III | 50mL | 4℃ |
| HAM Reaction Buffer | Solution IV | 1.2mL | -20℃ |
| LB Buffer 3 | Solution V | 50mL | 4℃ |
| Biotin Reaction Buffer | SolutionVI | 15μL | -20℃ |
| Streptavidin HRP | Solution VII | 5μL | -20℃ |
| Reagent name (4rnxs) | Reagent number | Volume (formal) | Storage temperature |
| LB Buffer 1 | Solution I | 8.2mL | 4℃ |
| Blocking Reagent | Solution II | 50μL | -20℃ |
| LB Buffer 2 | Solution III | 8.2mL | 4℃ |
| HAM Reaction Buffer | Solution IV | 220μL | -20℃ |
| LB Buffer 3 | Solution V | 8.2mL | 4℃ |
| Biotin Reagent Buffer | SolutionVI | 3μL | -20℃ |
| Streptavidin HRP | Solution VII | 20μL | -20℃ |
FAQ
Product Receipt Confirmation
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The insufficient volume of Solution II in the ABE (for WB) kit is due to the evaporation of some solvent ethanol, which can be supplemented with anhydrous ethanol to the corresponding volume; After receiving the product, some are in a dry powder state with only solutes. It is recommended to use and prepare it now.
All products can be transported at room temperature for a short period of time without affecting the quality and effectiveness of the products. Aims product related after-sales support provides unconditional returns and exchanges due to quality issues.
The product can be stored at -20 ℃ for long-term use and at 4 ℃ for short-term use.
If there is any loss, damage, or leakage of reagents, do not use them and contact after-sales service.
The StrePDAvin HRP reagent, due to its small packaging size, can be directly added to the corresponding laboratory incubation liquid and diluted proportionally before use.
Understand the experimental steps
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If there are any problems or operational errors in any step of the reagent kit, you can contact the after-sales service. Some steps can be paused and restarted, and the operability is strong.
Some of the parameters in the reagent kit are test parameters, please optimize them according to actual operating conditions for specific effects.
Product after-sales knowledge
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If the product reagent kit is lost, damaged, or erroneous due to human factors, resulting in the inability to continue the experiment, the user of the product shall bear the responsibility, and the product owner may provide corresponding support and assistance to the purchaser of the reagent kit.
The final use effect of the product is related to experimental factors such as personal use of antibodies and enrichment of captured beads. The interpretation right of the final product belongs to our company.
Introduction of Amperex Biotechnology Co., Ltd.:
Amperex Biotechnology Co., Ltd. has a very good reputation in the field of protein qualitative and quantitative sequencing such as MRM/SRM, iTRAQ, CoIP MS (protein interactions), acetylation/phosphorylation protein post-translational modifications, etc.
Five reasons to choose Amperex Biotechnology Co., Ltd
1. Committed to building a better proteomics research platform and providing better proteomics technologies
2. Extraction experience of multiple sample proteins (non model biological species such as corals, persimmons, bronchoalveolar lavage fluid, etc.)
3. Systematize laboratory management system and strictly control the quality process
4. Short project cycle, high-quality to meet customer needs
5. One on one responsibility system, with dedicated technical support for each project
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