Full protein palmitoylation ALK-16 click chemistry kit

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

The ALK-16 click chemistry kit for whole protein palmitoylation is used to detect palmitoyl sites/target protein palmitoyl by WB/MS method after processing the whole protein with this kit.

Product Details

Item No.	AM10818
Product Name	Palmitoylation ALK-16 click chemistry kit for WB/MS\|Full protein palmitoylation ALK-16 click chemistry kit
Product advantages:	Full protein palmitoylation ALK-16 click chemistry kitAM10818 was developed for the detection of palmitoyl modification sites in most studies on whole proteins/specific proteins. After processing the whole protein with this reagent kit, WB/MS method was used to detect the palmitoyl sites of the whole protein/target protein palmitoyl detection.
Product Features:	This kit can be used for WB/MS palmitoyl site detection of whole protein/target protein. One kit meets your various experimental needs, with easy to operate and error free results that can be repeated after repeated testing
Operation steps:	Marking closed capture detection
Product storage:	Reference kit composition table
Reagent name_20rnxs:	Palm Probe,4SB Buffer,Blocking Reagent 1,Blocking Reagent 2,CUAAC Buffer,2SB Buffer,Capture Beads,Washing Buffer,Reducing Buffer,Blocking Reagent 3
Reagent No. 20rnxs:	Solution I,Solution II,Solution III,Solution IV,Solution V ,SolutionVI,SolutionVII,SolutionVIII,SolutionIX,SolutionX
Volume_Form_20rnxs:	170μL,20mL,520μL,270μL,520μL,22mL,1270μL,27mL,2520μL,270μL
Storage temperature_20rnxs:	-20℃,-20℃,-20℃,-20℃,-20℃,-20℃,4℃,-20℃,-20℃,-20℃
Reagent name_4rnxs:	Palm Probe,4SB Buffer,Blocking Reagent 1,Blocking Reagent 2,CUAAC Buffer,2SB Buffer,Capture Beads,Washing Buffer,Reducing Buffer,Blocking Reagent 3
Reagent ID: 4rnxs	Solution I,Solution II,Solution III,Solution IV,Solution V ,SolutionVI,SolutionVII,SolutionVIII,SolutionIX,SolutionX
Volume_Form_4rnxs:	30μL,3.5mL,100μL,50μL,100μL,4mL,250μL,5mL,500μL,50μL
Storage temperature: 4rnxs	-20℃,-20℃,-20℃,-20℃,-20℃,-20℃,4℃,-20℃,-20℃,-20℃

Kit composition:

Reagent name (20rnxs)	Reagent number	Volume (formal)	Storage temperature
Palm Probe	Solution I	170μL	-20℃
4SB Buffer	Solution II	20mL	-20℃
Blocking Reagent 1	Solution III	520μL	-20℃
Blocking Reagent 2	Solution IV	270μL	-20℃
CUAAC Buffer	Solution V	520μL	-20℃
2SB Buffer	SolutionVI	22mL	-20℃
Capture Beads	SolutionVII	1270μL	4℃
Washing Buffer	SolutionVIII	27mL	-20℃
Reducing Buffer	SolutionIX	2520μL	-20℃
Blocking Reagent 3	SolutionX	270μL	-20℃

Reagent name (4rnxs)	Reagent number	Volume (formal)	Storage temperature
Palm Probe	Solution I	30μL	-20℃
4SB Buffer	Solution II	3.5mL	-20℃
Blocking Reagent 1	Solution III	100μL	-20℃
Blocking Reagent 2	Solution IV	50μL	-20℃
CUAAC Buffer	Solution V	100μL	-20℃
2SB Buffer	SolutionVI	4mL	-20℃
Capture Beads	SolutionVII	250μL	4℃
Washing Buffer	SolutionVIII	5mL	-20℃
Reducing Buffer	SolutionIX	500μL	-20℃
Blocking Reagent 3	SolutionX	50μL	-20℃

FAQ

Product Receipt Confirmation
The insufficient volume of Solution II in the ABE (for WB) kit is due to the evaporation of some solvent ethanol, which can be supplemented with anhydrous ethanol to the corresponding volume; After receiving the product, some are in a dry powder state with only solutes. It is recommended to use and prepare it now.

All products can be transported at room temperature for a short period of time without affecting the quality and effectiveness of the products. Aims product related after-sales support provides unconditional returns and exchanges due to quality issues.

The product can be stored at -20 ℃ for long-term use and at 4 ℃ for short-term use.

If there is any loss, damage, or leakage of reagents, do not use them and contact after-sales service.

The StrePDAvin HRP reagent, due to its small packaging size, can be directly added to the corresponding laboratory incubation liquid and diluted proportionally before use.

Understand the experimental steps
If there are any problems or operational errors in any step of the reagent kit, you can contact the after-sales service. Some steps can be paused and restarted, and the operability is strong.

Some of the parameters in the reagent kit are test parameters, please optimize them according to actual operating conditions for specific effects.

Product after-sales knowledge
If the product reagent kit is lost, damaged, or erroneous due to human factors, resulting in the inability to continue the experiment, the user of the product shall bear the responsibility, and the product owner may provide corresponding support and assistance to the purchaser of the reagent kit.

The final use effect of the product is related to experimental factors such as personal use of antibodies and enrichment of captured beads. The interpretation right of the final product belongs to our company.

Introduction of Amperex Biotechnology Co., Ltd.:

Amperex Biotechnology Co., Ltd. has a very good reputation in the field of protein qualitative and quantitative sequencing such as MRM/SRM, iTRAQ, CoIP MS (protein interactions), acetylation/phosphorylation protein post-translational modifications, etc.

Five reasons to choose Amperex Biotechnology Co., Ltd

1. Committed to building a better proteomics research platform and providing better proteomics technologies

2. Extraction experience of multiple sample proteins (non model biological species such as corals, persimmons, bronchoalveolar lavage fluid, etc.)

3. Systematize laboratory management system and strictly control the quality process

4. Short project cycle, high-quality to meet customer needs

5. One on one responsibility system, with dedicated technical support for each project