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Hangzhou Startech Biotechnology Co., Ltd
Taq DNA polymerase
NegotiableUpdate on 05/06
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Overview
Taq DNA polymerase is a heat stable DNA polymerase cloned from Thermus aquaticus, with 5 '→ 3' DNA polymerase activity and 5 '→ 3' exonuclease activity. This product has undergone strict purification and is free from contamination by endonucleases and exonucleases. It is suitable for routine molecular biology experiments such as PCR amplification and DNA labeling.
Product Details
Taq DNA polymerase (conventional type)
UA070125
Product Description
Taq DNA polymerase is a heat stable DNA polymerase cloned from Thermus aquaticus, with 5'3 'DNA polymerase activity and 5'3' exonuclease activity. This product has undergone strict purification and is free from contamination by endonucleases and exonucleases. It is suitable for routine molecular biology experiments such as PCR amplification and DNA labeling.
Product Features
high specificityOptimized buffer system ensures amplification specificity
High amplification efficiencyDNA fragments that can expand up to 5kb in length
thermal stabilityHalf life>40 minutes at 95 ℃
Strict quality control: No endonuclease/exonuclease contamination
ready-to-useProvide 2 x pre mixed buffers containing Mg ² ⁺
technical parameters
| parameter | specification |
|---|---|
| Definition of Activity | The enzyme required to catalyze the incorporation of 10 nmol dNTP into acid insoluble precipitates at 74 ℃ for 30 minutes |
| Optimal pH | 8.0-9.0 (Tris HCl buffer system) |
| Storage conditions | -Store at 20 ℃ (50 mM Tris HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 8.0) |
| Unit packaging | 500U/tube, 1000U/tube |
Scope of application
Conventional PCR amplification
DNA fragment labeling
Preparation of sequencing template
Mutation detection analysis
Usage recommendations
Recommended reaction system: 25-50 μ L
Typical amplification procedure: 94 ℃ for 3 minutes (94 ℃ for 30s, 55 ℃ for 30s, 72 ℃ for 1min/kb) x 30 cycles at 72 ℃ for 5 minutes
Suggested primer length: 18-25bp
Recommended primer concentration: 0.1-0.5 μ M
Precautions
This product is only for scientific research use
Please mix thoroughly before use
Avoid repeated freeze-thaw cycles
Recommend using the matching Buffer
Regarding Starter, a domestic antibody expert:
Hangzhou Startech aims to provide high-quality products and research and development services for the global life science industry, including antibodies, proteins, reagent kits, and more. Relying on multiple development platforms: recombinant monoclonal antibodies, recombinant mouse monoclonal antibodies, rapid mouse monoclonal antibodies, recombinant protein development platforms (E. coli, CHO, HEK293, InsectCells), it has officially passed the EU 98/79/EC certification ISO9001 certification ISO13485
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