Extraction free one-step RT qPCR kit (SYBR Green)

Extraction free one-step RT qPCR kit (SYBR Green)
UA079018

Product Description

This product is an innovative one-step RT qPCR detection kit that does not require nucleic acid extraction. It uses the SYBR Green fluorescent dye method and can quickly detect RNA targets from various samples (including cells, tissues, blood, etc.) without the need for pre RNA extraction and purification steps.

Product Features

• No extraction operation requiredDirectly add the sample lysate for RT qPCR reaction

• high sensitivityCan detect RNA templates as low as 10 copies

• Broad spectrum compatibilitySuitable for various RNA targets such as viral RNA, mRNA, lncRNA, etc

• rapid detectionThe whole process only takes 60-90 minutes

• Excellent amplification efficiencyPCR efficiency reaches 90-110%

• high specificityEquipped with optimized HotStart Taq DNA polymerase

Kit components

• 2× One Step RT-qPCR Mix

• RNase Inhibitor

• 50× ROX Reference Dye

• DEPC-treated Water

• 
  

technical parameters

application field

• Rapid detection of viral RNA (such as SARS-CoV-2)

• Gene expression analysis

• Transgenic testing

• Pathogen diagnosis

• scientific research experiment

Experimental Procedure

1. Sample lysis processing (5 minutes)

2. Preparation of reaction system (5 minutes)

3. RT qPCR reaction (60 minutes)

4. Result Analysis

Precautions

1. This product is only for scientific research purposes

2. Avoid repeated freeze-thaw cycles

3. The experimental operation needs to be conducted in an RNase free environment

4. Recommend using the matching positive control

Regarding Starter, a domestic antibody expert:
Hangzhou StartechWe aim to provide high-quality products and research and development services for the global life science industry, including antibodies, proteins, reagent kits, and more. Relying on multiple development platforms: recombinant monoclonal antibodies, recombinant mouse monoclonal antibodies, rapid mouse monoclonal antibodies, recombinant protein development platforms (E. coli, CHO, HEK293, InsectCells), it has officially passed the EU 98/79/EC certification ISO9001 certification ISO13485