Determination of Organic Acids by TSKgel ODS-100V Chromatography Column

Determination of Organic Acids by TSKgel ODS-100V Chromatography Column

Research Background

Organic acids are widely present in food, beverages, pharmaceuticals, and biological samples, and their types and contents directly affect product flavor, quality, and

Physiological activity. For example, citric acid and malic acid in food determine the sweetness and sourness; Oxalic acid, fumaric acid, and other substances in medicine are key indicators for quality control.

High performance liquid chromatography（HPLC is the mainstream technology for organic acid analysis, and the reverse phase C18 chromatographic column has become the core tool for organic acid detection due to its excellent separation performance.TOSOHselectTSKgel ODS100VinvertEstablish a high-performance separation and analysis method for organic acids using a C18 chromatographic column, with the following specific conditions:

Determination of Organic Acids by TSKgel ODS-100V Chromatography Column

Analysis conditions

Chromatographic column:TSKgel ODS-100V（4.6 mm I.D×25 cm ，5μm）

Mobile phase:0.1% H ₃PO₄

Current Speed:1.0 mL/min

Injection volume:10 ML

Detector:UV 210nm

Column temperature:40 ℃

Analysis results

1. oxalic acid(0.1 g/L)2. L-tartaric acid (0.5 g/L)3. formic acid(1.0 g/L)

4. L-Malic acid (1.0 g/L)5. L-Ascorbic acid (0.1g/L)6. lactic acid(1.0 g/L)

7. acetic acid(1.0 g/L)8. maleic acid(0.01g/L)9. citric acid(1.0 g/L)

10. succinic acid(1.0 g/L)11. Fumaric acid(0.025 g/L)12. acrylic acid(0.1 g/L)

13. propionic acid(2.0 g/L)14. Glutaric acid(1.0 g/L)15. itaconic acid(0.025 g/L)

Determination of Organic Acids by TSKgel ODS-100V Chromatography Column

conclusion

TOSOH'sTSKgel ODS-100V, as a classic reverse phase C18 column, exhibits unique advantages in organic acid separation:

· Moderate retention of strongly to weakly polar organic acids with symmetrical peak shapes

· Acid resistant mobile phase，Excellent stability

· achievableSimultaneous quantitative analysis of multiple organic acids in complex matrices

· With multiple particle size specifications, it can meet the needs of different scenarios.

Product Item Number

Determination of Organic Acids by TSKgel ODS-100V Chromatography ColumnGreen Baicao Technology Development Co., Ltd. is a technology-based company engaged in the development, technical services, and technical training of chromatography, purification, natural product extraction, and separation technologies. It specializes in representing domestic and foreign purification media, fillers, chromatography columns, laboratory instruments and equipment, consumables, reagents, and dynamic axial compression column systems for production. The company is located in the Zhongguancun Science and Technology Park in Beijing, which is densely populated with high-tech enterprises. Green Grass Company provides * services to scientific researchers and production plants in the fields of natural product extracts, traditional Chinese medicine, western medicine, biopharmaceuticals, food, environmental monitoring, petrochemicals, new materials, etc. with its technology and products.