T4 DNA Ligase T4 principle carrier and others

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

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Overview

T4 DNA Ligase is isolated and purified from Escherichia coli expressing the T4 DNA Ligase gene after induction. It catalyzes the phosphodiester bond binding reaction between the 5 '- phosphate end and the 3' - hydroxyl end of adjacent DNA strands. This enzyme catalyzes the connection of smooth or sticky end DNA, repairing single strand breaks in double stranded DNA, RNA, and DNA/RNA hybridization. $r $nT4 DNA Ligase T4 principle carrier and others

Product Details

Q 4 DNA ligase

T4 DNA Ligase T4 principle carrier and others

Catalog Number:42017

Composition and packaging of reagent kit:

compose	50U(42017-50)	100U(42017-100)	300U(42017-300)
Q 4 DNA ligase	50U	100U	300U
10 ´Ligation Buffer	40ml	80ml	120ml

Concentration:5U/ml

Storage and transportation temperature:-20℃（保存6Months)

Product Description:Q 4 DNA ligaseIt comes from expressingQ 4 DNA ligaseThe gene of Escherichia coli was isolated and purified after induction. It catalyzes adjacentDNAThe chain's5’-Phosphate terminal and3’The hydroxyl end undergoes a phosphodiester bond binding reaction. This enzyme catalyzes smooth or sticky endsDNAConnect and repair double linksDNATheRNATheDNA/RNASingle chain cleavage in hybridization.

Scope of application：（1）DNAFragments and carriersDNAQuick connection;

（2）Restricting the cloning of enzyme digested fragments;

（3）WillDNAFragments andLinkerorAdaptorconnect.

Enzyme storage buffer：

20mM Tris-HCl (pH7.5)，0.1 mM EDTA，1mM DTT，50 mM KCl，50% glycerol.

10×Ligation Buffer：

400 mM Tris-HCl，100 mM MgCl₂，100 mM DTT，5 mM ATP(PH7.8)Other components.

Suggest connecting the reaction system:(with10 μlTaking the reaction system as an example)

	Standard reaction tube	Positive control tube
Vector	ml	ml
PCRproduct	nml **	/
Comparison insertionDNAfragment	/	1ml
T4 DNAligase(5U/ml)	2～5U	2～5U
10×Ligation Buffer	1ml	1ml
Add deionized water to the final volume	10ml	10ml

Setting of reaction cycle：

Adhesive end connection:22^oCreaction10minute(Can be extended as needed1hour); It's also possible16^oCreaction1-5Hour.

Flat end connection:22^oCreaction2Hour or16^oCOvernight?

T4 DNA Ligase T4 principle carrier and others