DNA Quick Ligation Kit T4 principle carrier and others

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

This kit can quickly complete DNA fragment ligation reactions at room temperature (22 C) for 10 minutes. The connected product can be directly converted. Can be used for DNA fragment cloning, library construction, TA cloning, Linker ligation, etc. $r $nDNA Quick Ligation Kit T4 principle carrier and others

Product Details

DNA Quick Ligation Kit

DNA Quick Ligation Kit T4 principle carrier and others

Catalog Number:42110

Catalog Number	packaging unit
42110-20	20 times
42110-60	60 times

Components	42110-20	42110-60
2 xQuick Ligation Buffer	100 ml	300 ml
Q 4 DNA ligase, 5 U /ml	100 U	300 U

Product storage:-20°C Save to avoid excessive repeated freeze-thaw cycles.

Product Description:This reagent kit can be used at room temperature (22 ° C)°C) Under 10 minute conditions, quickly complete the DNA fragment adhesion or flat end ligation reaction. The connected product can be directly converted. Can be used for DNA fragment cloning, library construction, TA cloning, Linker ligation, etc.

characteristic：The connection reaction can be completed in 10 minutes with fast, simple, and adhesive ends.

Operation steps:

1. The reaction proceeds according to the following system:

5 ml	2 xQuick Ligation Buffer
ml	Vector DNA(30-60ng）
ml	Insert DNA (3 times the molar amount of vector, with a molar ratio of 3:1 between insertion and vector)
ml	sterile water
1 ml（5 Weiss Units/ml)	Q 4 DNA ligase
Final Volume	10ml

Generally, T4 DNA Ligase is added at the end, thoroughly mixed, and the liquid can be collected by instant centrifugation to the bottom of the tube.

2. 22°C connection takes 10 minutes (usually completed on a PCR instrument).

Usually recommended 22°C connection takes 5-10 minutes, and>3kb long segment connection can be extended to 30 minutes. Not recommended for more than 30 minutes, as exceeding it may reduce the number of conversions.

3. Cool on ice and then directly convert or store in -20 ° C°CIf stored at -20°C， Before conversion, it should be re dissolved on ice and thoroughly mixed before conversion.

Instructions:

1. Do not heat inactivate T4 DNA ligase before conversion, otherwise it will reduce conversion efficiency.

2. DNA fragments with a volume greater than 4mBy adding 2 x Quick Ligation Buffer, the total reaction volume can be increased to 20ml， In this way, larger volumes of DNA fragments can be added to the connection system.

DNA Quick Ligation Kit T4 principle carrier and others