Supply of Cell pH Detection Kit

Precautions for using the Cell pH Detection Kit (Blue Fluorescence): 1. The reagents in the spiral cap micro reagent tube should be briefly centrifuged before opening the lid to collect the liquid on the inner wall of the lid to the bottom of the tube and avoid liquid spillage when opening the lid. Before use, take out the product and warm it back to room temperature, then perform a brief centrifugation to concentrate the DMSO solution at the bottom of the tube. 2. The pH probe is a DMSO solution, which solidifies at room temperature during low winter temperatures and is highly prone to adhere to the tube wall and suction head wall. Attention should be paid to heating and dissolving, and the suction head should also be preheated in the incubator, otherwise it is easy to solidify again on the inner wall of the suction head and cause loss.

Storage temperature: 2-8 ℃, away from light

Precautions

1. The cell pH detection kit (blue fluorescence) can be stored at -20 ℃ if not used for a long time. Avoid repeated freezing and thawing.

2. Probe A is a DMSO solution, which solidifies when the temperature is low in winter and easily adheres to the tube wall and suction head wall. Note that it needs to be heated and melted, and after becoming liquid, centrifuge to the bottom of the tube before opening the lid.

Validity period: 6 months

Detection method: Flow cytometer//laser confocal/fluorescence microscope

Applicable sample: Cells

Product Application: Flow Cytometer//Laser Confocal/Fluorescence Microscope

Instrument preparation

1. Flow cytometry, laser confocal microscopy, or fluorescence microscopy

2. Centrifuge

3. Pipette

4. Refrigerator

5. Ice box

Reagent preparation

1. PBS buffer 2. HBSS 3. (Optional) Nitrogen sodium salt (related product BB-94002)

Preparation of consumables

1. Centrifugal tube

2. Suction head

3. Disposable gloves

1. Before opening the screw cap micro reagent tube, please briefly centrifuge the reagent to collect the liquid on the inner wall of the cap to the bottom of the tube to avoid liquid spillage when opening the cap. Before use, take out the product and warm it back to room temperature, then perform a brief centrifugation to concentrate the DMSO solution at the bottom of the tube.

2. The pH probe is a DMSO solution, which solidifies at room temperature during low winter temperatures and is highly prone to adhere to the tube wall and suction head wall. Attention should be paid to heating and dissolving, and the suction head should also be preheated in the incubator, otherwise it is easy to solidify again on the inner wall of the suction head and cause loss.

　　The cell pH detection kit detects the pH value inside cells by using pH indicators or pH sensing probes. There are usually several methods available：

Fluorescence probe method:

Some cell pH assay kits use fluorescently labeled pH sensing probes that can bind to hydrogen ions (H ⁺) inside cells. The fluorescence intensity of the probe will change according to the pH value. By measuring changes in fluorescence, the pH value inside the cell can be calculated.

For example, commonly used fluorescent probes include SNARF-1 and BCECF, which exhibit changes in fluorescence intensity and emission wavelength under different pH environments. These probes are analyzed using devices such as laser confocal microscopy or fluorescence spectrophotometer.

PH indicator method:

Another common method is to use pH indicators, which change color at different pH. This method is typically used for observation under a microscope or in combination with flow cytometry. For example, pH indicators such as phenol red can exhibit different colors in cell culture media with changes in pH.

Flow cytometry (FACS) method:

Using flow cytometry combined with fluorescent probes to measure the pH value of individual cells. This method can perform high-throughput pH detection in cell populations.

Potentiometer method:

The potential law uses pH electrodes or ion selective electrodes (ISE) to directly measure the potential changes inside cells and calculate the pH value.

common problem:

Q1: What is the principle usually used for cell pH detection?