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Cell hypoxia detection, reactive oxygen species detection, oxygen consumption rate detection kit
NegotiableUpdate on 05/06
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Overview
Cell hypoxia detection, reactive oxygen species detection, oxygen consumption rate detection kit supply, cell hypoxia detection kit RU (dpp) 3cl2- red fluorescence usage precautions: 1. Before opening the screw cap micro reagent tube, please centrifuge briefly to collect the liquid on the inner wall of the cap to the bottom of the tube, to avoid liquid spillage when opening the cap. 2. Cell processing requires careful handling to avoid human damage to cells as much as possible. Not all cell types consume enough oxygen for detection. 4. It is necessary to use a transparent black culture plate specifically designed for fluorescence cultivation to reduce light interference between each well during detection.
Product Details
Reactive oxygen species detection kit/Oxygen consumption rate detection kit/Cell hypoxia detection kit (supplied to various parts of China such as Shanghai/Hangzhou/Shenzhen)
Introduction to Cell Hypoxia Detection Kit
storage temperature
-Avoid light at 20 ℃
Precautions
1. The probe should be sealed at -20 ℃ and stored away from light;
2.Cell hypoxia detection kit RU (dpp) 3cl2 red fluorescenceAvoid repeated freezing and thawing.
Validity period
6 months
detection method
1. Fluorescence microscope 2. Flow cytometer 3. Laser confocal microscopy
Applicable samples
1. Suspended cells 2. Adhering cells
Product Application
Research on cellular metabolism
Instrument preparation
1. Fluorescent enzyme-linked immunosorbent assay (ELISA) reader
Corresponding wavelength filters and temperature controllers must be equipped.
2. Centrifuge
3. Pipette
4. Refrigerator
5. Ice box
Reagent preparation
PBS buffer or HBSS
Preparation of consumables
1. Black transparent fluorescent special 96 well culture plate
2. Centrifugal tube
3. Suction head
4. Disposable gloves
Cell hypoxia detection kit RU (dpp) 3cl2 red fluorescence usage precautions
1. Before opening the screw cap micro reagent tube, please briefly centrifuge the reagent to collect the liquid on the inner wall of the cap to the bottom of the tube to avoid liquid spillage when opening the cap.
2. Cell processing requires careful handling to avoid human damage to cells as much as possible.
Not all cell types consume enough oxygen for detection.
4. It is necessary to use a transparent black culture plate specifically designed for fluorescence cultivation to reduce light interference between each well during detection.
Cell hypoxia detection is mainly used to evaluate the physiological and biochemical reactions of cells under hypoxic conditions. Cells produce reactive oxygen species (ROS) under hypoxic conditions and may respond to hypoxic stress by increasing oxygen consumption rate. Common methods for detecting cellular hypoxia include reactive oxygen species detection and oxygen consumption rate detection.
Reactive Oxygen Species (ROS) detection: Reactive Oxygen Species (ROS) are short-lived molecules produced by cells when subjected to oxidative or hypoxic stress, including superoxide anions (O ₂⁻), * (H ₂ O ₂), hydroxyl radicals (• OH), etc. ROS has a significant impact on the redox state of cells and plays important roles in various physiological and pathological processes such as cell growth, signal transduction, aging, inflammation, and tumors.
Oxygen consumption rate detection: Cells need to consume oxygen for cellular respiration during physiological activities. The Oxygen Consumption Rate (OCR) of cells is an important indicator of cellular metabolic activity, reflecting the rate at which cells utilize oxygen. Under hypoxic conditions, cells adapt to low oxygen environments by altering metabolic pathways, such as activating anaerobic metabolism. Therefore, changes in oxygen consumption rate are often used to evaluate the adaptive response of cells under hypoxic conditions.
common problem:
Q1: What is the principle commonly used for detecting cellular hypoxia?
A1: Common principles include using fluorescent probes that are sensitive to oxygen concentration. Under hypoxic conditions, the fluorescence intensity or color of the probe will undergo specific changes, indirectly reflecting the oxygen level inside the cell.
Q2: Why use a red fluorescent probe (such as RU (dpp) 3cl2)?
A2: Red fluorescence has stronger penetration and is less affected by cell spontaneous fluorescence interference, which can reduce background signals and make detection results more accurate and sensitive.
Q3: What are the main application scenarios for detecting cellular hypoxia?
A3: Widely used in basic research related to tumor research (simulating the hypoxic microenvironment inside tumors), cardiovascular diseases (such as myocardial ischemia), neurodegenerative diseases, and metabolism.
Q4: What is the difference between cell oxygen consumption rate (OCR) detection and hypoxia probe detection?
A4: OCR directly measures the rate of oxygen consumption by cell respiration, reflecting metabolic activity; The hypoxia probe detects the instantaneous oxygen partial pressure in the cell's surrounding environment, directly indicating the state of hypoxia.
Q5: How to interpret the results of cell hypoxia detection?
A5: The stronger the fluorescence signal, the more severe the degree of hypoxia. A normoxic control group needs to be set up for comparison, and the level of hypoxia can be quantitatively analyzed by the difference in fluorescence intensity or proportion.
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