Sugar meter/refractometer calibration

The working principle of digital sugar meters and digital refractometers (hereinafter referred to as instruments) is to use the principles of refraction and reflection of light,
An instrument that automatically displays measurement values through signal acquisition and analysis processing technology of photosensitive components. This instrument usually consists of a light source
Composed of a refractive prism, temperature measurement (control) system, and digital display system, it can quickly measure transparency, translucency, dark color, and stickiness
The sugar content and refractive index of various liquids such as thick ones.

Sugar meter/refractometer calibrationThe detailed methods are as follows: (JJG (Jing) 73-2021)
1Calibration method for digital sugar meter

Preparation of standard materials:
Prepare sugar water solutions of different concentrations as standard substances, which should have high chemical purity (not less than 99.5%), stable quality, and similar physical and chemical properties to the tested sample.

Perform calibration:
Insert the digital sugar meter into the sugar solution, measure according to the operating instructions, and record the measurement results.
Evaluate the measurement performance of digital sugar meters by calculating accuracy and precision.
According to the evaluation results, adjust the parameters of the digital sugar meter and re measure the sugar solution to ensure the accuracy of calibration.

Record calibration results:

Record the calibration results in detail in the calibration report. In addition, for certain models of digital sugar meters, such as instruments with specific calibration buttons, calibration can be performed through the following simplified steps:
Press the Read button to turn on the device.
Drip irrigation absorbs 0.2-0.3ml of pure water, drips it into the drip port, and covers it with a light shielding cover.
Press the CAL key for 2 seconds to display CRL, then press the CAL key again to complete calibration and display END.

IICalibration method of digital refractometer
Select standard substances:
Select standard materials with different refractive indices to prepare liquid or solid standard samples. These standard substances should have high purity, stable density, and good chemical stability.

Perform calibration:
Place the digital refractometer in the standard sample, measure according to the operating instructions, and record the measurement results.
Evaluate the measurement performance of a digital refractometer by calculating its accuracy and precision.
According to the evaluation results, adjust the parameters of the digital refractometer and re measure the standard sample.

Record calibration results:
After calibration is completed, record the calibration results in detail in the calibration report.
The specific calibration method for digital refractometers may vary depending on the model.

Grey board calibration method:
Place the gray board on the measuring table of the digital refractometer and adjust the intensity of the light source to make the display close to 100.
Adjust the zero adjustment screw to make the measurement reading 0.
Repeat multiple measurements to obtain a stable average reading.
Adjust the zero calibration screw again to make the measurement reading 0 and complete the calibration.

Standard measurement object calibration method:
Place the standard measuring object on the measuring table of the digital refractometer and adjust the intensity of the light source to make the display close to 100.
Measure the refractive index n of the standard measuring object and record the reading.
Move the standard measuring object away and adjust the calibration screw to make the reading equal to the standard value.
Repeat multiple measurements to obtain a stable average reading.

IIISugar meter/refractometer calibrationPrecautions
During the calibration process, it should be ensured that there is no interference from stray light in the measurement area, and the surface of the standard substance or sample should be kept clean to avoid fingerprints or dust affecting the calibration accuracy.
Before calibration, the instrument should be preheated to ensure stability.
During calibration, the operating instructions of the instrument should be followed to avoid improper operation that may cause damage to the instrument or inaccurate calibration results.
In summary, through strict calibration procedures and selection of standard substances, the measurement accuracy and stability of digital sugar meters and digital refractometers can be ensured.

Company Background Introduction:
Ze Heng The advantage of Huapu in metrology calibration, verification, and confirmation is that it focuses on providing quality control services for biopharmaceuticals. It is very familiar with some special instruments, and its main team comes from pharmaceutical companies and instrument manufacturers, who are familiar with instrument and GMP regulations. For example, our team has specially developed a fluorescence quantitative PCR instrument calibration device to replace imports, which greatly reduces the cost of fluorescence quantitative/qualitative PCR instrument calibration (far lower than other peers); We have developed an internal calibration protocol for the cell counter; Developed internal calibration procedures for cell recovery instruments; The calibration procedure of gel electrophoresis scanner was developed; We have developed integrated wireless probes for lower and higher temperatures required for temperature verification, reducing the cost of users using probes extensively and replacing imports; There are many more examples, as we all come from a biological background, we pay attention to the process requirements of every biological user, focus on biopharmaceutical instruments and equipment, and strive to "go with quality and do instrument and equipment quality control" in order to make biopharmaceuticals safer.
We work closely with other units in terms of services, including mandatory inspections, special inspections, or services that cannot be done, to help customers send and collect items on their behalf, saving time, effort, and convenience.
Ze Heng Huapu is a professional service provider dedicated to providing laboratory instrument calibration, instrument and equipment validation, and factory validation for the biopharmaceutical industry. We provide pharmaceutical clients with one-stop quality control outsourcing services for QC/QA/engineering/validation/production departments.