Calibration of gel imager

Calibration of gel imager is an important step to ensure its stable performance and accurate data. The following is a universalCalibration of gel imagerMethod:

JJF 1530 regulation, namely JJF 1530-2015 Calibration Specification for gel Imaging System, is a national metrological technical specification issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. The following is a detailed interpretation of the regulation:

1、 Overview of Regulations

• Procedure name: JJF 1530-2015 Calibration Specification for gel Imaging System

• Published by: General Administration of Quality Supervision, Inspection and Quarantine of China

• Implementation date: September 15th, 2015

• Centralized by: National Technical Committee on Biometry

• Main drafting unit: China Institute of Testing Technology

• Participating drafting units: China Institute of Metrology, etc

2、 Scope of application of the regulations

This procedure is applicable to the calibration of gel imaging system for imaging nucleic acid electrophoresis gel and protein electrophoresis gel. Other types of gel imaging systems can refer to this procedure.

3、 Regulations content

This procedure mainly specifies the calibration method, calibration items and calibration requirements of gel imaging system to ensure the accuracy and reliability of gel imaging system. The specific content includes but is not limited to the following aspects:

1. Calibration method: it specifies the calibration steps and procedures of the gel imaging system, including preparation before calibration, operation requirements during calibration, and data processing after calibration.

2. Calibration items: specify the items that need to be calibrated for the gel imaging system, such as grayscale level, UV excitation spectrum, etc. These items can comprehensively reflect the performance parameters of the gel imaging system.

3. Calibration requirements: specific requirements are proposed for the calibration results of the gel imaging system. For example, the calibration error should be controlled within a certain range to ensure the accuracy and stability of the system.

4、 Meaning of regulations

The release and implementation of JJF 1530-2015 Calibration Specification for gel Imaging System is of great significance for improving the accuracy and reliability of gel imaging system. It standardizes the calibration methods and requirements of the gel imaging system, and provides a reliable calibration basis for researchers and experimenters. At the same time, the implementation of this procedure will also help promote the further development and application of gel imaging technology.

5、 Implementation suggestions for regulations

In order to ensure the accuracy and reliability of the gel imaging system, it is recommended that scientific researchers and experimental personnel calibrate the gel imaging system in strict accordance with JJF 1530-2015 Calibration Specification for gel Imaging System when using the gel imaging system. At the same time, it is recommended to regularly maintain the gel imaging system to extend its service life and improve its performance.

In conclusion, JJF 1530-2015 Calibration Specification for gel Imaging System is an important national metrological technical specification, which is of great significance for improving the accuracy and reliability of gel imaging system. Researchers and experimenters should strictly follow the requirements of this regulation for calibration and maintenance work.

Company Background Introduction:
Ze Heng The advantage of Huapu in metrology calibration, verification, and confirmation is that it focuses on providing quality control services for biopharmaceuticals. It is very familiar with some special instruments, and its main team comes from pharmaceutical companies and instrument manufacturers, who are familiar with instrument and GMP regulations. For example, our team has specially developed a fluorescence quantitative PCR instrument calibration device to replace imports, which greatly reduces the cost of fluorescence quantitative/qualitative PCR instrument calibration (far lower than other peers); We have developed an internal calibration protocol for the cell counter; Developed internal calibration procedures for cell recovery instruments; The calibration procedure of gel electrophoresis scanner was developed; We have developed integrated wireless probes for lower and higher temperatures required for temperature verification, reducing the cost of users using probes extensively and replacing imports; There are many more examples, as we all come from a biological background, we pay attention to the process requirements of every biological user, focus on biopharmaceutical instruments and equipment, and strive to "go with quality and do instrument and equipment quality control" in order to make biopharmaceuticals safer.
We work closely with other units in terms of services, including mandatory inspections, special inspections, or services that cannot be done, to help customers send and collect items on their behalf, saving time, effort, and convenience.
Ze Heng Huapu is a professional service provider dedicated to providing laboratory instrument calibration, instrument and equipment validation, and factory validation for the biopharmaceutical industry. We provide pharmaceutical clients with one-stop quality control outsourcing services for QC/QA/engineering/validation/production departments.