South American fetal bovine serum

South American fetal bovine serum, 2mg/mL 1mL price for transportation and storage on dry ice, stored at -80 ℃ to avoid repeated freeze-thaw cycles, with a shelf life of six months.

　　South American fetal bovine serum， 2mg/mL1mL Price Product and Features:

SDS polyacrylamide gel electrophoresis (SDS-PAGE) is the main method for denaturation and separation of proteins by electrophoresis at present, but it is tedious to prepare various solutions separately, so Tianze Gene has developed this product. It has the following characteristics:

1. One stop shop, ready to use, users do not need to prepare various ingredients separately, which is very convenient.

2. Safety: Minimize the possibility of experimental personnel coming into contact with powdered acrylamide.

3. Flexible and separate provision of acrylamide and methacrylamide facilitates the preparation of SDS-PAGE in various proportions and concentrations, meeting the requirements for separating proteins of different sizes.

4. Use an improved concentrated gel buffer solution, as it contains dyes, the prepared concentrated gel appears blue, making it easier to identify the sample wells during loading.

5. After electrophoresis, it can be directly used for experiments such as staining, silver staining, Western hybridization, etc.

　　South American fetal bovine serumIntroduction:

1. When using this product, a 30% acrylamide solution needs to be prepared first: Add 146mL of self prepared deionized water to the plastic bottle containing 60g of acrylamide dry powder provided in this product, shake thoroughly until dissolved to obtain 200mL of 30% acrylamide solution (shake by hand for about 10-20 minutes).

2. When using this product again, a 30% solution of acrylamide methacrylamide (19:1) should be prepared: different experiments require the addition of different proportions of methacrylamide. For SDS-PAGE electrophoresis, the ratio of acrylamide to methylene bisacrylamide is generally around 19:1, as the pore size of the PAGE gel is small and the resolution is high at this time. The preparation method is to add 3g of formaldehyde bisacrylamide dry powder to 200mL of 30% acrylamide solution prepared in the previous step (note: formaldehyde bisacrylamide dry powder has neurotoxicity, avoid inhaling the powder). The prepared 30 ° C solution should be stored in the dark at 4 ° C and used up within one month.

3. Estimate the volume of concentrated glue and separation glue to be prepared based on the area of the flat plate and the thickness of the glue. And determine the concentration of concentrated gel and separation gel based on the size of the protein to be separated

4. Prepare 10% APS (ammonium persulfate): Weigh 0.1 grams of APS dry powder into 1 mL of deionized water and shake to dissolve. The prepared 10% APS solution can be stored at 4 ℃ for one week.

5. Preparation of separation gel: In a 2mL triangular flask, add water, 30% solution, and 4 times separation gel buffer solution according to the following table. The following is the dosage for preparing 10mL of glue. For larger volumes, the dosage of each component needs to be increased proportionally. Acrylamide solution has neurotoxicity and must be operated with gloves.

100mL of culture medium for rat tracheal epithelial cells

Mouse bladder smooth muscle cell culture medium 100mL

Fraser broth enrichment solution FB1 supporting reagent specifications: 2x5 tubes. Usage: Add one tube each of reagent A and reagent B to 225ml (026080) to prepare FB1 enrichment solution.

Brucella broth specification: 250g Purpose: Used for culturing Campylobacter jejuni and observing its motility. (GB、ISO)

Recombinant protein of interleukin 1 alpha (IL1 alpha) English name: Recombinant Interleukin-1 Alpha (IL1a)

Recombinant Bone Morphogenetic Protein Receptor 1B (BMPR1B)

Recombinant Family With Sequence Similarity 5, Member C (FAM5C)

CoC1 (human ovarian cells) 5 × 106 cells/bottle × 2

WISH (human amniotic membrane cells) 5 × 106 cells/bottle × 2

PC-12 (rat renal pheochromocytoma cells) 5 × 106 cells/bottle × 2

Milk English name: MDA-MB-157

English name of mouse fibroblast: 3T3TWO-PRO 3 (equivalent to TO-PRO 3)

Cy3NS 酸 Cy3NS acid

Cal-520- Biocytin Conjugate

IP Receptor (mouse) Blocking Peptide (1 ea) Prostacyclin Receptor|PGI2 Receptor|Prostaglandin I2 Receptor； IP Receptor (mouse) Blocking Peptide

19(R)-hydroxy Prostaglandin B2 (500 ug) 9-oxo-15S，19R-dihydroxy-prosta-5Z，8(12)，13E-trien-1-oic acid； 19(R)-OH PGB2； 19(R)-hydroxy Prostaglandin B2

Octyl-α-ketoglutarate (5 mg) 2-oxo-1-octyl ester-pentanedioic acid； α-KG octyl ester； Octyl-α-ketoglutarate

(-)-WIN 55，212-3 (mesylate) (25 mg) [(3S)-2，3-dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1，2，3-de]-1，4-benzoxazin-6-yl]-1-naphthalenyl-methanone， methanesulfonate； (-)-WIN 55，212-3 (mesylate)

SR 1555 (hydrochloride) (1 mg) 1-(4-((4’-(1，1，1，3，3，3-hexafluoro-2-hydroxypropan-2-yl)-[1，1’-biphenyl]-4-yl)methyl)piperazin-1-yl)ethanone， monohydrochloride； SR 1555 (hydrochloride)

5-trans Prostaglandin D2 (100 mg) 9。 alpha。 ，15S-dihydroxy-11-oxo-prosta-5E，13E-dien-1-oic acid； 5，6-trans PGD2； 5-trans Prostaglandin D2

2-Pyridylamide oxime (1 g) N-hydroxy-2-pyridinecarboximidamide； Picolinic Acid Amidoxime； 2-Pyridylamide oxime

SB 431542 (10 mg) 4-[4-(1，3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]-benzamide； SB 431542