Human ELISA kit

The human ELISA kit company is selling products: AFP (A4) alpha fetoprotein monoclonal antibody (detection) ARFIP1 ADP ribosylation factor binding protein 1 antibody AHR aromatic hydrocarbon receptor antibody ATRN attractant antibody AKAP95 protein kinase A anchoring protein 95 antibody ASBT parietal membrane sodium dependent bile salt transporter protein antibody

Note: Our company provides free testing services for reagent kits. If you need other testing kits, please consult the sales staff.

Product Name:Human ELISA kit

English name: IL-2R Elisa Kit

Product Code: GOY-0002E

Specification: 96T/48T

保存； 2-8℃。

96T can produce 94 specimens, 2 standard controls, and 84 samples

48T can produce 47 specimens -1 standard control -42 samples

Detection purpose: Used for detecting samples such as plasma, serum, and related fluids. For example, specimens such as lavage fluid, cerebrospinal fluid, serum, plasma, urine, pleural and peritoneal fluid, cell culture supernatant, tissue homogenate, etc

Species detected: rats, mice, guinea pigs, hamsters, nude mice, rabbits, pigs, dogs, monkeys, horses, cows, potatoes, deer, sheep, chickens, ducks, fish, humans, and other animals and plants.

It has the following advantages:

1、 Efficient, sensitive, and specific antibodies;

2、 Stable repeatability and reliability;

3、 Solid phase carriers with good adsorption performance, low blank value, and high transparency at the bottom of the pores;

4、 Suitable for various specimen types such as serum, plasma, tissue homogenate, cell culture supernatant, urine, etc;

5、 The cost-effectiveness is very good, saving experimental expenses.

Performance:

1) Accuracy: The correlation coefficient R value between standard linear regression and expected concentration is greater than or equal to 0.9900.

2) Sensitivity: Detection concentration is less than 1.0 pg/ml.

3) Specificity: Does not cross react with other soluble structural analogues.

4) Repeatability: The coefficient of variation within and between plates is less than 15%.

5) Storage: Store at 2-8 ℃, away from light and moisture.

6) Validity period: 6 months

Measurement steps:

1. Sample addition

1. Except for the bedding, samples need to be added at a 45 degree angle

2. The sample volume should be accurate

3. Sample should be added to the bottom of the pipe, not to the wall of the pipe

4. No bubbles should be generated during sample addition

2. Warm bath

After adding the specimen and the conjugate, they should be immediately placed in a water bath at the specified reaction temperature.

2. The ELISA plates should not be stacked together.

To avoid evaporation, the board should be covered or placed flat in a metal wet box with wet gauze at the bottom.

After adding the substrate, the reaction time and temperature are usually not strictly required. If the room temperature is above 20 ℃, the ELISA plate can be placed in the dark on the experimental bench for occasional observation. When the control tube develops color appropriately, the enzyme reaction can be terminated.

3. Washing

1. Washing is not a reaction step in the ELISA process, but it is the key to determining the success or failure of the experiment.

2. The purpose is to wash away substances in the reaction solution that have not bound to the solid-phase antigen or antibody, as well as interfering substances that have non-specific adsorption on the solid-phase carrier during the reaction process.

4. Reading board

1. The color of the negative control is extremely light, and visual colorimetry can generally be used in qualitative testing.

If the results are measured using an ELISA reader, the accuracy depends on the flatness and transparency of the ELISA plate bottom, the quality of the ELISA reader, and the algorithm of the software.

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Operation steps:

The sandwich method generally involves the following steps:

Fix specific antibodies onto plastic wells and wash away excess antibodies after completion;

Add the test sample, and if the sample contains the antigen to be tested, it will specifically bond with the antibody on the plastic well disk;

Wash away excess test samples, add another antigen specific primary antibody, and bond with the test antigen;

Wash away excess unbound primary antibodies, add secondary antibodies with enzymes, and bond with the primary antibodies;

Wash away excess unbound secondary antibodies, add enzyme substrate to color the enzyme, and read the color result with naked eye or instrument;

Indirect method, the general operating steps are:

Fix the known antigen onto a plastic well plate and wash away excess antigen after completion.

Add the test sample, and if the sample contains the primary antibody to be tested, it will specifically bond with the antigen on the plastic well disk.

Wash away excess test samples, add secondary antibodies with enzymes, and bond with the primary antibody to be tested.

Wash away excess unbound secondary antibodies, add enzyme substrate to color the enzyme, and measure the absorbance value in a plastic dish using an instrument to evaluate the content of colored end products and measure the content of the antibody to be tested.

The operation steps of competition law are:

Fix specific antibodies onto plastic wells and wash away excess antibodies after completion

Add the test sample to enable specific binding between the test antigen in the sample and the antibody on the plastic well disk

By adding an antigen with an enzyme, this antigen can also specifically bond with antibodies on the plastic well disk. Due to the limited number of antibodies fixed on the plastic well disk, the more antigens in the sample, the fewer immobilized antibodies that can be bound to the enzyme antigen. In other words, both antigens compete to bond with antibodies on the plastic well disk, which is the origin of the so-called competitive method.