Probe PCR detection kit

Product Features:

This product is a highly sensitive fluorescent quantitative PCR product developed based on primers designed for conservative regions.

The principle is mediated by a pair of primers, which can rapidly amplify specific gene (DNA) fragments in vitro in animals and plants. After n thermal cycles of amplification, the number of specific genes contained in the amplification product is (1+E) n (0) of the original template number

Transportation: Low temperature

Save: Negative 20 degrees

Validity period: One year

Delivery time: 2-3 weeks

Notes:

1. Basic program;

2. Amplification temperature and extension temperature;

3. Reaction time;

4. Number of cycles;

5. Preparation of PCR reaction solution;

6. The basic principles of PCR technology;

7. Reaction kinetics of PCR;

8. PCR amplification products;

9. PCR reaction system and reaction conditions.

Usage:

1、 Dilute the standard curve sample (using 6 10 fold dilutions of 10E2-10E7 copies/μ L as an example). Due to the high concentration of the standard substance, the following dilution operations must be carried out in a separate area and must not contaminate the sample or other components of this kit.

1. Mark 6 centrifuge tubes, namely 7, 6, 5, 4, 3, and 2.

2. Add 45 μ L of fluorescent PCR template dilution solution using a core gun tip, the same applies below.

3. Add 5 μ L of positive control (concentration of 1 × 10E8 copy/μ L, provided by the reagent kit) to tube 7, shake thoroughly for 1 minute, and obtain a standard curve sample of 1 × 10E7 copy/μ L. Put it on ice for later use.

4. Change the gun head and add 5 μ L of 1 × 10E7 copy/μ L positive control (diluted in the previous step) to tube 6. Shake thoroughly for 1 minute to obtain a standard curve sample of 1 × 10E6 copy/μ L. Put it on ice for later use.

5. Change the gun head and add 5 μ L of 1 × 10E6 copy/μ L positive control (diluted in the previous step) to tube 5. Shake thoroughly for 1 minute to obtain a standard curve sample of 1 × 10E5 copy/μ L. Put it on ice for later use.

6. Repeat the above operation until obtaining standard curve samples with 6 dilutions. Put it on ice for later use.

2、 Preparation of Sample DNA

7. Purify the DNA of the sample using a self selected method. This product is compatible with the vast majority of nucleic acid purification products on the market.

If there are N samples, N+2 sample extractions are required, with the extra one used as a positive control tube for sample preparation and the other as a negative control tube for sample preparation.

3、 Set up qPCR reaction (20 μ L system, conducted in the sample preparation room)

9. If quantitative analysis is performed and only one replicate is performed, label N+9 PCR tubes, including N+2 for the N+2 samples obtained in the previous step, 1 for PCR negative control (using water as a template), and 6 for the standard curve. If qualitative analysis is performed and only one repetition is made, label N+4 PCR tubes, including N+2 for the N+2 samples obtained in the previous step, 1 for PCR negative control (using water as a template), and 1 for PCR positive control.

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Calsequierstrin 2 (CASQ2) salt berberiberamine

Histatin 1 (HTN1) berberine salt

Junctophilin 2 (JPH2) salt root alkaloid

Junctophilin 3 (JPH3) salt irinotecan

Intersectin 2 (ITSN2) salt cultured Hengbin

Janus Kinase 3 (JAK3) Yangmei Glycoside

　　Probe PCR detection kitPrice Janus Kinase 2 (JAK2) Yangmei extract

Kisspeptin Receptor (KISS1R), Thistle Extract (1,3-Dicaffeoylquinine), Type A Influenza Virus H5N1 Agglutinin, H5N1 Hemagglutinin

Human Cytomegalovirus UL49 Protein Antibody HCMV UL49

Hepatonuclear factor 1 β/HNF-1 β antibody HNF1 beta

HHLA3 protein antibody HHLA3

HHIP like protein 2 antibody HHIPL2

Type A influenza virus H3N2-M2 protein antibody H3N2 Matrix Protein 2

HSH2D抗体HSH2D

Hirudin antibody

White blood cell antigen A antibody HLA Class 1 ABC

Human papillomavirus type 16 E7 antibody HPV16 E7

Notes:

① The order of adding reagents should be consistent to ensure that the incubation time for all reaction plate wells is the same.

② Use clean plastic containers to prepare detergent.

③ Incubate according to the time, amount, and sequence indicated in the instructions of the chicken hemoglobin (HB) ELISA detection kit.

④ Substrate A should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light and should be avoided from prolonged exposure to light. Avoid contact with hands, toxic. After the experiment is completed, the OD value should be read immediately.

⑤ When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried. Do not directly put the absorbent paper into the ELISA reaction well to absorb water.

⑥ Use disposable suction tips to avoid cross contamination, and avoid using sample dispensers with metal parts when suctioning termination solution and substrates A and B.

⑦ The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.

⑧ Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored.

⑨ Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.