Single cell injection

Single Cellome Unit SU10 is capable of delivering substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells. It can also be sampled on single cells.
It is suitable for use with inverted optical microscopes and stereomicroscopes. Microscope is not included in SU10.

Advantages of SU10

-Single cell targeting, directly delivered to the nucleus or cytoplasm
-Sampling nanodots from the nucleus or cytoplasm of cells
-Small cell damage
-Automatic, high-speed, high success rate

Detailed introduction

Single cell targeting, directly delivered to the nucleus or cytoplasm

When observed under a microscope, select the cells to be delivered and deliver the substance to the nucleus or cytoplasm of the target cell.

Easily control the XY axis position in the software and automatically control the delivery Z-axis position.

Deliver FITC labeled dextran solution (molecular weight 70000) into HeLa cells.

Small cell damage

Nano straw is a type of glass straw with a tip diameter as small as tens of nanometers, which can minimize damage to cells as much as possible.

Delivery with high cellular vitality, enabling live single-cell analysis.

Nano pipette tip under electron microscope

Automatic, high-speed, high success rate

SU10 can automatically perform cell surface detection, insertion, and delivery to cells. This process takes approximately 10 seconds and has a success rate of 90%. (Experiment conducted by Yokogawa Electric)

With SU10, operations previously performed manually by experienced researchers have become much easier.

Example of Targeted Cells

Cell lines (HeLa, HEK293, CHO, MDCK, HepG2, etc.)

Primary cell culture (neurons, liver cells, etc.)

Stem cell lines (iPS cells, ES cells, etc.)

Immune cell lines (T cells, Ba/F3 cells, etc.)

Plant cells with cell walls

Example of Injectable Substances

CRISPR-Cas9 (Cas9 RNP)

Proteins (antibodies, GFP, etc.)

Nucleic acid (single stranded DNA, etc.)

fluorescent agent

Application example

characteristic

1. Minimally invasive nanopipette

SU10 can inject genes, drugs, and other substances into the target location of a single cell, and extract intracellular substances. Compared to micro scale pipettes used for performing such tasks, the SU10 uses a nano pipette with a needle outer diameter of approximately 100 nanometers (nm), making it one of the smallest pipettes available in the field of biological research. The needle of this nanopipette is much smaller than the cells analyzed by SU10, with only slight invasiveness, thus enabling single-cell analysis of live cells.

2. Improve efficiency through automated analysis

SU10 can automatically perform a series of steps, starting from detecting the cell surface, until the cell surface penetrates, and then injecting or suctioning substances into or from the cell, all of which still require manual operation by skilled researchers to date. By using SU10, it is easy to perform live cell injection and aspiration operations.

Q&A

Q1. What is the difference between it and a microinjector?

A1. The use of nano straws in SU10 can significantly reduce cell damage, as the outer diameter of its needle is less than 1/10 of that of a microinjector.
Through the automatic detection function on the cell surface, high success rates of insertion can be achieved at the expected cell depth.
The injection action is controlled by electric control, not pneumatic or hydraulic control.

Q2. How is it different from traditional transfection methods such as transfection reagents and electroporation?

A2. SU10 can inject substances into designated cells.
SU10 can directly inject reagents into the cytoplasm or nucleus.

Q3. What is the difference between it and electroporation?

A3. In addition to the differences from traditional transfection methods mentioned above, there is no need to suspend cells during injection due to the automatic detection of cell surface.

Q4. What is the maximum cell injection volume?

A4. Expected to soar several tens of liters per second (fL) (1 fL=1 × 10)-15L)。
The injection amount can be changed through software settings.
*The injection amount may vary depending on the solute and carrier.

Q5. Is the nano straw disposable?

A5.Yes, but a nanopipette can deliver at least 50 cells *.
*HeLa cell experiment conducted by Yokogawa Electric