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Life Science Business | Yokogawa Electric (China) Co., Ltd

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CSU-X1 laser confocal scanning unit

NegotiableUpdate on 05/07
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Overview

The CSU-X1 laser confocal scanning unit is the * tool for live cell imaging, with an imaging capability of 2000 frames per second. The use of micro lens enhanced Nipkow disk scanning technology can significantly reduce photobleaching and phototoxicity. The device has filter wheels with six filter positions, which improves excitation power efficiency and signal-to-noise ratio, supports dual camera and bright field imaging modes, and reduces CO2 emissions by 40%. Its high efficiency makes it a powerful tool for live cell imaging.

Product Details

Faster, brighter, and more features

CSU-X1 laser confocal scanning unitIt is a high-speed model of the CSU series and a tool for live cell imaging.

Scanning speed up to 2000 fps

Micro lens enhanced Nipkow disk scanning

Filter wheel with six filter positions (high-end model)

Replaceable dichroic mirror block and emission filter

3 configurations: single camera model, dual camera model, and clear field of view model

CO2Reduce emissions by 40%


Principle of Micro Lens Enhanced Nipkow Rotary Scanning Technology

CSU-X1激光共聚焦扫描单元

CSU-X1 laser confocal scanning unitA Nipkow disk containing approximately 20000 pinholes and a disk containing the same number of microlenses (used to focus the excitation laser into each corresponding pinhole) are mechanically fixed with a motor, and when rotated, the field of view is scanned by a fast grating with approximately 1000 laser beams. The pinhole and micro lens patterns are designed exclusively by Yokogawa Electric to optimize grating scanning. Using CSU-X1 for multi beam scanning can not only improve scanning speed, but also significantly reduce photobleaching and phototoxicity, as multiple excitations only require lower levels of laser power on the sample to excite fluorescence.


Detailed introduction

faster

Scanning speed up to 2000fps

Yokogawa Electric's proprietary filter wheel with six filter positions moves to adjacent positions at a relatively fast speed of 33 milliseconds.




brighter

By using the newly developed beam shaping lens, the excitation power efficiency can be doubled*1Allowing the use of lower power lasers and reducing camera exposure time.

CSU-X1激光共聚焦扫描单元


By reducing background noise by one-third, the signal-to-noise ratio can be increased by three times*1Expand the ability of true low light imaging. By using highly efficient dichroic mirrors and emission filters, the image is significantly brighter*1.

CSU-X1激光共聚焦扫描单元




More features - Dual camera option * 1

Users can use two cameras to simultaneously image two different emission ranges, or selectively use one of the two cameras installed that is more suitable for the current experimental requirements. For each camera port, users can choose to install a high-speed filter wheel (optional). In addition to the standard C-mount adapter, adapters for 8X8 EMCCD cameras and F-mount cameras are also available (optional).

CSU-X1激光共聚焦扫描单元




More Features - Clear View Path Options * 2

It allows users to use a camera for confocal imaging through CSU-X1 and bright field (non confocal) imaging through a bypass optical path.

CSU-X1激光共聚焦扫描单元




More features - replaceable mirror block and replaceable filter

Easy to replace dichroic mirror block and emission filter.

CSU-X1激光共聚焦扫描单元

*1. It is necessary to use the commercially available dichroic mirror selected by the user for synchronous multi-color imaging.

*Due to spatial interference, the bright field option is not suitable for certain microscope settings. Please consult its applicability in advance.


High end model (6-digit filter wheel)

CSU-X1激光共聚焦扫描单元


High end model (12 position filter wheel)

CSU-X1激光共聚焦扫描单元


Basic model

CSU-X1激光共聚焦扫描单元


Host*1

Host+
Bright Vision*2

Host+
Dual cameras*2

Confocal scanning method

Micro lens enhanced Nipkow disk scanning

scanning speed

Selection: 1500-5000rpm (standard)
1500-10000rpm (high speed)*3

External Synchronization

Synchronize scanning speed through pulse signals
Input: TTL 300Hz~2KHz
Corresponding to the rotational speed of Nipkow turntable
1,500~10,000rpm*3

excitation wavelength

405~647nm

Another port

-

bright field

Dual cameras

dichroic mirror

Optional*4

Mirror switching

Automatic 3-channel
(replaceable dichroic mirror block)

Laser beam input

AFC connector (polished 8 degrees)

optical fiber

Standard single-mode polarization maintaining fiber of Yokogawa Electric Corporation*5

Filter wheel
(Launch side)

Filter wheel with six filter positions

Emission filter

Optional*4

Control Panel

Open/close laser shutter

external control

Through the RS-232C interface of the control unit

Microscope bracket

C mount adapter

Operating environment

15-40 ℃/20-75% relative humidity

energy consumption
(Host)

24VDC maximum 1A

energy consumption
(AC adapter)

Input: 100-240VAC ± 10%, 50 or 60Hz ± 3Hz, maximum 75W
Output: 24VDC up to 2.5A

External dimensions*6

175(W)×328.5(H)×301.5(L)mm

175(W)×328.5(H)×301.5(L)mm

175(W)×328.5(H)×301.5(L)mm

Weight*7

8.9kg

11.7kg

13.0kg


*1. Provide a control unit (for the filter wheel) and a filter wheel.
*Provide two control units (one filter wheel and one bright field) and one filter wheel.
*3 options available
*4 does not include filters (excitation filter, emission filter, dichroic mirror). If necessary, please consult in advance.
*5 are provided with each CSU-X1 host.
*6 does not include protruding parts. Including the filter wheel.



Host*1

Host+
Bright Vision*2

Host+
Dual cameras*2

Confocal scanning method

Micro lens enhanced Nipkow disk scanning

scanning speed

Selection: 1500-5000rpm (standard)
1500-10000rpm (high speed)*3

External Synchronization

Synchronize scanning speed through pulse signals
Input: TTL 300Hz~2KHz
Corresponding to the rotational speed of Nipkow turntable
1,500~10,000rpm*3

excitation wavelength

405~647nm

Another port

-

bright field

Dual cameras

dichroic mirror

Optional*4

Mirror switching

Automatic 3-channel
(replaceable dichroic mirror block)

Laser beam input

AFC connector (polished 8 degrees)

optical fiber

Standard single-mode polarization maintaining fiber of Yokogawa Electric Corporation*5

Filter wheel
(Launch side)

Filter wheel with 12 filter positions

Emission filter

Optional*4

Control Panel

Open/close laser shutter

external control

Through the RS-232C interface of the control unit

Microscope bracket

C-mount adapter

Operating environment

15-40 ℃/20-75% relative humidity

energy consumption
(Host)

24VDC maximum 1A

energy consumption
(AC adapter)

Input: 100-240VAC ± 10%, 50 or 60Hz ± 3Hz, maximum 75W
Output: 24VDC up to 2.5A

External dimensions*6

258(W)×329.8(H)×213.4(L)mm

259(W)×374.3(H)×248(L)mm

309.8(W)×329.8(H)×392(L)mm

Weight*7

7.8kg

10.6kg

12.2kg

*1. Provide a control unit (for the filter wheel) and a filter wheel.
*Provide two control units (one filter wheel and one bright field) and one filter wheel.
*3 options available
*4 does not include filters (excitation filter, emission filter, dichroic mirror). If necessary, please consult in advance.
*5 are provided with each CSU-X1 host.
*6 does not include protruding parts. Including the filter wheel.


Host*1

Host+
Bright Vision*2

Host+
Dual cameras*2

Confocal scanning method

Micro lens enhanced Nipkow disk scanning

scanning speed

Selection: 1500-5000rpm (standard)
1500-5000rpm (high speed)*3*4
1500-10000rpm (high speed)*3*4

External Synchronization

Optional*4

excitation wavelength

405~647nm

Another port

-

bright field

Dual cameras

dichroic mirror

Optional*5

Mirror switching

Manual Channel 1
(replaceable dichroic mirror block)

Laser beam input

AFC connector (polished 8 degrees)

optical fiber

Standard single-mode polarization maintaining fiber of Yokogawa Electric Corporation*6

Filter wheel
(Launch side)

-

Emission filter

Optional*5

Control Panel

Open/close laser shutter

external control

-*4

Microscope bracket

C mount adapter

Operating environment

15-40 ℃/20-75% relative humidity

energy consumption
(Host)

24VDC maximum 1A

energy consumption
(AC adapter)

Input: 100-240 VAC ± 10%, 50 or 60Hz, up to 75W
Output: 24VDC up to 2.5A

External dimensions*6

175(W)×328.5(H)×213.4(L) mm

259(W)×373(H)×213.4(L)mm

308.5(W)×328.5(H)×213.4(L)mm

Weight*7

7.5kg

10.0kg

10.0kg

*1. Provide a control unit (for the filter wheel) and a filter wheel.
*Provide two control units (one filter wheel and one bright field) and one filter wheel.
*3 options available
*4. The control unit is required for speed control and external synchronization.
*5 does not include filters (excitation filter, emission filter, dichroic mirror). If necessary, please consult in advance.
*6 is provided with each CSU-X1 host.
*7 does not include protruding parts.

Control unit/filter wheel

control unit

type

Used for 6-digit filter wheel (F1)

Used for 12 position filter wheel (F2)

Used for bright vision (B1)

Operating environment

15-40 ℃ 20-75% relative humidity

energy consumption

Input: 100-240VAC ± 10%, 50 or 60Hz, maximum 200VA

Overall dimensions
(mm)

213(W)×132(H)×438(L) mm

Weight (kg)

5.2 kg

5.2 kg

5.1 kg

6-position filter wheel for CSU-X1

Operating environment

15-40 ℃ 20-75% relative humidity

energy consumption

-

Overall dimensions

112(W)×226(D)×100(H)

weight

1.9 kg

12 position filter wheel for CSU-X1

Operating environment

15-40 ℃ 20-75% relative humidity


energy consumption

-


Overall dimensions

154(W)×98(D)×154(H)


weight

2.4 kg



Comparison between target product and reference product

CSU-X1激光共聚焦扫描单元


Target product: CSU-X1

Reference product: CSU22+filter wheel

Energy (MJ)

CO2Emissions (kg)

NOx emissions (g)

SOx emissions (g)

Energy (MJ)

CO2Emissions (kg)

NOx emissions (g)

SOx emissions (g)

Raw materials

2,335.5

132.5

337.1

130.1

2,610.0

148.0

374.9

147.1

component

10,510.2

494.5

1,423.4

452.8

15,329.2

728.1

2,061.7

671.7

Processing and assembly

1,542.9

68.7

214.1

56.8

2,259.8

100.7

313.6

83.1

logistics

2,717.7

185.5

210.8

471.1

3,211.7

218.9

252.3

553.9

consume

36,713.5

1,635.9

5,094.8

1,351.5

66,087.0

2,944.8

9,171.0

1,126.4

process

-569.9

-31.9

-75.7

-33.6

-612.8

-34.5

-81.2

-37.4

Total

53,249.8

2,485.3

7,204.4

2,428.8

88,884.8

4,106.0

12,092.2

3,851.5

Functional factors

40.1%

39.5%

40.4%

36.9%


Note: The value of reference products is calculated based on functional factors. Functional factor: 2.16