Simpsil Sugar RN Poria cocos specific column

The main components of Poria cocos include polysaccharides（70-90%）Triterpenoid compounds（0.5-3%）Dietary fiber (approximately)50%）Protein（1.5-3%）Minerals (calcium, potassium, magnesium, etc.) and small amounts of vitamins. The specific content is affected by factors such as origin, variety, and processing method, and needs to be comprehensively judged based on testing reports or literature data.

High performance liquid chromatography is currently the most commonly used and accurate method for the target components of Poria cocos. Inject the sample and reference solution separately into silica gel filled with secondary/tertiary amine bondsSimpSil Sugar-RNPoria cocos specific columnBy detecting with an evaporative light scattering detector and using the logarithmic equation of the external standard two-point method, it can be obtained.

SimpSilSugar-RNPoria cocos specific columnIt uses secondary/tertiary amine bonded silica gel as a filler, which can effectively achieve good separation effect and is in line with2025According to the requirements of the Chinese Pharmacopoeia, the number of theoretical plates calculated based on glucose peak should not be less than1500.

Sugar-RNOrdering information for Poria cocos special columns:

Excerpt from Poria cocos in the 2025 edition of the Chinese Pharmacopoeia:

Content determinationDetermine according to the high-performance liquid chromatography method (General Rule 0512).

Chromatographic conditions and system suitability testUsing secondary/tertiary amine bonded silica gel as a filler; Acetonitrile-Water（80：20）For the mobile phase; useEvaporative light scattering detector detection. The number of theoretical plates calculated based on the glucose peak should not be less than1500.

Preparation of reference solutionTake an appropriate amount of anhydrous glucose reference standard, accurately weigh it, and add water to make each one1mlcontain1.0mgThe solution is obtained.

Preparation of test solutionTake the powder of this product (sieved through the third sieve) approximately0.5gAccurately weighed, placed in a conical flask with a stopper, and precisely added0.5mol/LThe * solution25ml, sealing, ice water bath ultrasonic treatment (power)250WFrequency40kHzThe temperature is controlled within10～20℃）45Minutes, filtered. Precision suction of continuous filtrate1mlPlace it in a stoppered test tube and add it precisely1.0mol/LHydrochloric acid solution0.5ml, Vortex30Mix thoroughly in seconds and add precisely7.0mol/LThe ThreeFluoroacetic acid solution6.0ml, in110℃ hydrolysis6After hours, cool to room temperature and recover the solvent to dryness. Dissolve the residue in water and transfer it to10mlDilute to the mark in a volumetric flask, shake well, filter, and take the continuous filtrate to obtain.

Measurement methodAccurately extract the reference solution separately5μlThe10μlCompared to the test solution5μlInject it into the liquid chromatograph, measure it, and calculate the logarithmic equation using the external standard two-point method.

This product is calculated as dry and contains Poria cocosB-（13）-Glucan as anhydrous glucose（C₆H₁₂O₆）Calculate, not less than50%.