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Shanghai Yihe Applied Biotechnology Co., Ltd
Second-generation sequencing
NegotiableUpdate on 05/06
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Overview
Second generation sequencing, also known as bisulfite amplification sequencing (BSAS). Yihe Biological DNA Methylation Sequencing can perform high-throughput methylation resequencing analysis on target intervals, accurately calculate the methylation rate of each site, and perform quantitative analysis at single base resolution, with high cost-effectiveness.
Product Details
Second-generation sequencingIntroduction:
Epigenetics is currently one of the hotspots in molecular biology research, and DNA methylation sequencing is an important part of epigenetics, which is one of the main epigenetic modifications of genomic DNA. DNA methylation refers to a modification method in which a methyl group is transferred to a cytosine base under the catalysis of DNA methyltransferases (DNMTs). It mainly occurs in regions rich in dinucleotide CpG islands. Under normal circumstances, CpG islands exist in a non methylated form (active form), while DNA methylation can lead to gene expression silencing. The abnormal activity of DNMTs is closely related to diseases. It plays a crucial role in maintaining normal cellular function, transmitting genomic genetic imprints, embryonic development, and human tumorigenesis.
Shanghai Yihe Biotechnology treated the target fragment with bisulfite and amplified it using multiplex PCR. Barcodes and sequencing universal adapters were added and used in Illumina X10Second-generation sequencingThe platform performs high-throughput sequencing of PCR products and uses bioinformatics methods to accurately quantify the methylation status of methylation sites within the target region, significantly reducing research costs while completing target area detection.
Hi MethylSeq combines sulfite conversion and targeted amplicon high-throughput sequencing technology to achieve precise quantitative analysis of multi segment and multi site methylation. This method is applicable for the methylation study of the target fragment of interest, and further confirms the positive sites selected for whole genome methylation research in large samples.
Bisulfite treatment is the gold standard for genomic DNA processing in DNA methylation sequencing. It is a chemical process that can cause DNA damage and it is difficult to achieve 100% conversion efficiency while maintaining DNA integrity. A certain balance needs to be struck between the two. Select 3 non CpG island sequences outside the Hi MethylSeq target area as internal controls to accurately evaluate the conversion rate of the samples.
