Cell line STR identification

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

Cell line STR identification refers to the genetic characteristics of a cell line established through STR (short tandem repeat) mapping. After the genetic characteristics of a cell line are established, the cell line can be regularly tested to prevent misidentification or cross contamination. Cell line STR identification is a professional service launched since 2014. It is one of the earliest companies in China to provide genetic quality identification services for cell line STR. So far, it has years of experience in cell STR identification services and more than ten years of STR typing experience.

Product Details

Cell line identification refers to the genetic characteristics of a cell line established through STR (short tandem repeat) mapping. After the genetic characteristics of a cell line are established, the cell line can be regularly tested to prevent misidentification or cross contamination.

When is cell line identification necessary?

When establishing or acquiring a new cell line;
At the beginning/end of a cell assay project
Before cell cryopreservation, or when cells have been continuously cultured for 2-3 months
Before publishing an article or applying for research funding
When the cell line exhibits instability or results differ significantly from expectations
When using more than one cell line in the laboratory, it is best to identify all cell lines first to rule out the possibility of cross contamination

How to conduct cell line identification?

The identification of cell lines is currently mainly based on the standards of the International Commission on Identity. According to this standard, multiple fluorescence PCR technology can be used to detect 8 STR loci and 1 sex determination locus in human sources. The following table shows the genetic information of these loci.

STR Alleles on gene loci can be distinguished by the different copy numbers of repeat sequences within the amplification region, and can be identified by fluorescence detection after capillary electrophoresis separation. Then, through a certain calculation method, the obtained STR typing results can be compared with the cell STR database to calculate the name of the cell line to which the sample belongs or the possible cross contamination cell line.

DSMZ tools were used for cell line alignment, which included 2455 cell line STR data from ATCC, DSMZ, JCRB, and RIKEN databases. If the cells to be tested are not included in the above cell bank or if they are newly established cell lines, they cannot be compared. Users need to compare their cell typing results with other databases on their own.