SYTO 9/PI dual staining kit for live/dead bacteria

Product Introduction

SYTO 9/PI dual staining kit for live/dead bacteria（SYTO 9/PI Live/Dead Bacterial Double Stain Kit）It is a convenient and easy to operate reagent kit that utilizesSITE 9Green nucleic acid dye and propidium iodide（PI）Red fluorescent nucleic acid dye is used for detecting bacterial vitality and is suitable for a large number of bacterial species,

The working principle of this reagent kit is:SITE 9andPIThe spectral characteristics and ability to penetrate healthy bacterial cells vary. When used alone,SITE 9Can label all bacteria within the groupBacteria with intact and damaged membranes; On the contrary,PICan only penetrate into damaged membranes,PIInsertion can causeSITE 9The decrease in staining fluorescence occurs when two dyes are added to the system. Therefore, through an appropriate proportionSITE 9andPIThe mixed staining shows that bacteria with intact membrane structures exhibit green fluorescence, while bacteria with damaged membrane structures exhibit red fluorescence. The maximum excitation and emission wavelengths of the two dyes are, respectively480/500nm（SITE 9）And490/635nm（PI）. The background has almost no fluorescence. This kit is compatible with fluorescence microscopes, fluorescence photometers, fluorescence enzyme-linked immunosorbent assay (ELISA) analyzers, flow cytometers, or other fluorescence detection instruments.

SYTO 9/PI dual staining kit for live/dead bacteria

Product Composition

[Note]componentCFluorescence microscopy detectionIt will only be applied in the method, please refer to the precautions for specific usage3)

Kit specifications (calculated based on recommended reagent dilution ratios and single test volumes):Preservation and transportation methods:-20℃Store away from light, with a validity period of one year. Ice pack transportation.

Fluorescence microscopy detection：1mlAddition of bacterial solution3μlDye mixture（SYTO 1.5μl +PI 1.5μl）, can be done40Independent testing.

Fluorescent enzyme-linked immunosorbent assay (ELISA) detection：2mlAdd sterile water12μlDye mixture（SYTO 6.0μl +PI 6.0μl）, prepared as2×Working fluid. according to100μlAddition of dye mixture100μlThe proportion of bacterial liquid used can be200Independent testing.

Flow cytometry detection：2mlAddition of bacterial solution6μlDye mixture（SYTO 3.0μl +PI 3.0μl）, can be done20Independent testing.

Usage(The following steps are only used as examples to guide researchers in staining their own bacterial samples.)