SPF grade pathogen quality control multiplex qPCR joint detection kit for large and small mice (national standard virus selection test six items)

SPF level rat and mouse national standard virus selection test six item qPCR kit

(Probe method)

SPF. M/R-OV-001 50 T/ 盒

Biowing^®SPF grade rat and mouse national standard selected virus qPCR joint detection kit,SPF level rat and mouse national standard virus selection test six item qPCR kit，adoptTaqman fluorescence quantitative PCR technology is designed for rapid and highly sensitive screening of common SPF laboratory mice and rats in multiple sample types6Designed for planting viruses.

This reagent kit adopts a one-step methodRT-qPCR， Design 6 sets of primer probes for detection in two channels: FAM (lymphocyte choriomeningitis virus LCMV (RNA), mouse norovirus MNV (RNA), rat coronavirus RCV (DNA)), and HEX (mouse poxvirus/foot-and-mouth virus Ect (DNA), mouse encephalomyelitis virus TMEV (RNA), and multi tumor virus Poly (DNA))6Seed virus, another set of primer probesCY5 channel detects internal reference RNA. This reagent kit has the following characteristics:

sensitiveHigh detection sensitivity,10 copies/μ L can be effectively detected.

efficientSingle tube can be detected at once6 types of viruses.

stableFull process closed pipe operation without cross contamination.

reliableContains internal reference control to avoid false negatives.

convenientEasy to operate, one-step detection.

Transportation and Storage Conditions

Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.

A BOX:

B BOX:

Note:

（1) Positive quality control (PC1) includes three types of viral nucleic acids or plasmid DNA containing specific fragments, namelyLymphocytic choriomeningitis virusLCMV、 Mouse pox virus/footless virus Ect, internal reference RNA.

（2) Positive quality control (PC2) includes three types of viral nucleic acids or plasmid DNA containing specific fragments, namelyMouse NorovirusMNV、 Mouse encephalomyelitis virus TMEV, internal reference RNA.

（3) Positive quality control (PC3) includes three types of viral nucleic acids or plasmid DNA containing specific viral fragments, namelyrat coronavirus,RCVRCV、 Polyomavirus Poly, internal reference RNA.

【 Precautions 】

Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.

1. Suggest partitioning operation

（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.

（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.

（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.