SPF grade pathogen quality control multiplex qPCR joint detection kit for large and small mice (eight mandatory tests for national standard viruses)

SPF level rat and mouse national standard virus mandatory eight item qPCR kit

(Probe method)

SPF. M/R-RV-001 50 T/ 盒

Biowing^®SPF grade pathogen quality control multiplex qPCR joint detection kit for large and small mice (eight mandatory tests for national standard viruses)，SPF level rat and mouse national standard virus mandatory eight item qPCR kit, usingTaqman fluorescence quantitative PCR technology is designed for rapid and highly sensitive screening of common SPF grade mice in multiple sample types8Designed for planting viruses.

This reagent kit adopts a one-step methodRT-qPCR， Design 8 sets of primer probes for detection in three channels: FAM (Reo-3 RNA for type III reovirus, SV RNA for Sendai virus, MHV RNA for mouse hepatitis virus), HEX (HANT RNA for Hantavirus, PVM RNA for mouse pneumonia virus, MVM DNA for mouse parvovirus), and ROX (KRV DNA for rat parvovirus, H-1 DNA for rat parvovirus)8Seed virus, another set of primer probesCY5 channel detects internal reference RNA.This reagent kit has the following characteristics:

sensitiveHigh detection sensitivity,10 copies/μ L can be effectively detected.

efficientSingle tube can be screened once8 types of viruses.

stableFull process closed pipe operation without cross contamination.

reliableContains internal reference control to avoid false negatives.

convenientEasy to operate, one-step detection.

Transportation and Storage Conditions

Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.

A BOX:

B BOX:

Note:

（1) Positive quality control (PC1) includes four types of viral nucleic acids or plasmid DNA containing specific fragments, namely Reo-3 type III reovirus, HANT Hantavirus, KRV rat parvovirus, and internal reference RNA.

（2) Positive quality control (PC2) includes four types of viral nucleic acids or plasmid DNA containing specific fragments, namely Sendai virus SV, mouse pneumonia virus PVM, rat parvovirus H-1, and internal reference RNA.

（3) Positive quality control (PC3) includes three types of viral nucleic acids or plasmid DNA containing specific fragments, namely mouse hepatitis virus MHV, mouse parvovirus MVM, and internal reference RNA.

【 Precautions 】

Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.

1. Suggest partitioning operation

（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.

（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.

（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.

2. other

（1) Due to the positive quality control of B BOX, in order to avoid contamination of the reagent by nucleic acid, A and B BOX should be stored separately.

（2) Each component should be centrifuged at low speed before use and carefully opened. Please gently invert and mix ten times before use to avoid foaming, and centrifuge briefly at low speed before use.

（3) When sealing or capping qPCR reaction plates, they need to be repeatedly compressed.

（4) Before amplification, the reaction tube is centrifuged at low speed for a short period of time to collect the liquid on the tube wall to the bottom.

（5) After covering the reaction tube lid or applying optical film, to avoid affecting the reading of fluorescence signals, please be careful not to make marks on the tube lid or film, or repeatedly rub with a scraper.