SPF grade multiple qPCR joint detection kit for quality control of pathogenic bacteria in rats and mice (eight mandatory tests for pathogenic bacteria according to national standards)

SPF grade rat and mouse national standard pathogen testing eight item qPCR kit

(Probe method)

SPF. M/R-RB-001 50 T/ 盒

Biowing^®SPF grade rat and mouse national standard required pathogen qPCR detection kit,SPF grade rat and mouse national standard pathogen testing eight item qPCR kit，Using highly sensitive and specific probe methodsQPCR technology is specifically designed for rapid and highly sensitive screening of SPF grade laboratory mice in various sample types8Designed for pathogenic bacteria.

This reagent kit usesTaqman fluorescence quantitative PCR technology was used to design 8 sets of primer probes for detection in three channels: FAM (Mycoplasma Ms, Taize pathogen Cp, Pseudomonas aeruginosa Pa), HEX (Corynebacterium cepacium Ck, Pasteurella pneumophila Pp, Bordetella bronchiseptica Bb), and ROX (Salmonella Ss, Klebsiella pneumoniae Kp)8Plant pathogenic bacteria, in addition1Primer probe set inCY5 channel detects internal reference DNA. This reagent kit has the following characteristics:

sensitiveHigh detection sensitivity,10 CFU can be effectively detected.

efficientSingle tube can be screened once8 types of pathogenic bacteria.

stableFull process closed pipe operation without cross contamination.

reliableContains internal reference control to avoid false negatives.

convenientEasy to operate, one-step detection.

Transportation and Storage Conditions

Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.

A BOX:

B BOX:

Note:

（1) Positive quality control (PC1) includes four types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments, namely Mycoplasma Ms, Corynebacterium Ck, Salmonella Ss, and internal reference DNA.

（2) Positive quality control (PC2) includes four types of pathogen nucleic acids or plasmid DNA containing specific fragments, namely Taize pathogen Cp, Pasteurella pneumophila Pp, Klebsiella pneumoniae Kp, and internal reference DNA.

（3) Positive quality control (PC3) includes three types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments, namely Pseudomonas aeruginosa Pa, Bordetella bronchiseptica Bb, and internal reference DNA.

【 Precautions 】

Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.

1. Suggest partitioning operation

（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.

（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.

（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.