SPF grade pathogen quality control multiplex qPCR joint detection kit for large and small mice (national standard pathogen selection test seven items)

SPF grade rat and mouse national standard pathogen selection test seven item qPCR kit

(Probe method)

SPF. M/R-OB-001 50 T/ 盒

Biowing^®SPF grade rat and mouse national standard selected pathogen qPCR detection kit,SPF grade rat and mouse national standard pathogen selection test seven item qPCR kit,Using highly sensitive and specific probe methodsQPCR technology is specifically designed for rapid and highly sensitive screening of SPF grade laboratory mice in various sample types7Designed for pathogenic bacteria.

This reagent kit usesTaqman fluorescence quantitative PCR technology was used to design 7 sets of primer probes for FAM，HEX，ROX three channel detection7A pathogenic bacterium and another set of primer probes are usedCY5 channel detects internal reference DNA. This reagent kit has the following characteristics:

Sensitivity:The detection sensitivity is high,10 CFU can be effectively detected.

Efficient:Single tube can be screened once7 types of pathogenic bacteria.

stable:Full process closed pipe operation without cross contamination.

Reliable:Contains internal reference genes to avoid false negatives.

Convenient:Easy to operate, one-step detection.

Transportation and Storage Conditions

Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.

A BOX:

B BOX:

Note:

（1) YangNature control（PC1) Contains four types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments.

（2) Positive quality control (PC2) includes three types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments.

（3) Positive quality control (PC3) includes three types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments.

【 Precautions 】

Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.

1. Suggest partitioning operation

（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.

（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.

（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.