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Shanghai Yihe Applied Biotechnology Co., Ltd
SPF grade multiple qPCR detection kit for common bacteria in rats and mice
NegotiableUpdate on 05/06
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Overview
Biowing The qPCR detection kit for common pathogenic bacteria in SPF grade rats and mice, as well as the multiplex qPCR detection kit for common pathogenic bacteria in SPF grade rats and mice, use highly sensitive and specific probe based qPCR technology. It is designed for rapid and highly sensitive screening of six common pathogenic bacteria in SPF grade laboratory mice and rats in multiple sample types. This reagent kit adopts Taqman fluorescence quantitative PCR technology, designs 6 sets of primer probes, and sets up two qPCR systems.
Product Details
SPF grade multiple qPCR detection kit for quality control of pathogens in rats and mice (6 common pathogen detection items)
SPF grade multiple qPCR detection kit for common bacteria in rats and mice
(Probe method)
SPF. M/R-CB-001 50 T/ 盒
Biowing®QPCR detection kit for common pathogenic bacteria in SPF grade rats and mice,SPF grade multiple qPCR detection kit for common bacteria in rats and miceUsing highly sensitive and specific probe qPCR technology, specifically designed for rapid and highly sensitive screening of common SPF laboratory mice and rats in multiple sample types6Designed for pathogenic bacteria.
This reagent kit usesTaqman fluorescence quantitative PCR technology was used to design 6 sets of primer probes and set up a two tube qPCR systemAdditionally, design a set of primer probes to detect internal reference DNA in the CY5 channel. This reagent kit has the following characteristics:
sensitiveHigh detection sensitivity,10 CFU can be effectively detected.
stableFull process closed pipe operation without cross contamination.
reliable：Contains internal reference control to avoid false negatives.
convenientEasy to operate, one-step detection.
Transportation and Storage Conditions
Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.
A BOX:
Component Name |
filling volume |
Biowing®Bacteria qPCR Reaction Mix |
275 μL×4pipe |
Biowing®Common Bacteria Primer&Probe Mix1 |
lyophilized powder2 tubes |
Biowing®Common Bacteria Primer&Probe Mix2 |
lyophilized powder2 tubes |
RNase Free Water |
1.1 mL x 2 tubes |
paraffin oil |
1.1 mL x 1 tube |
B BOX:
Component Name |
filling volume |
Positive quality control1（PC1） |
lyophilized powder2 tubes |
Positive quality control2（PC2） |
lyophilized powder2 tubes |
RNase Free Water |
1.1 mL x 1 tube |
Note:
（1) Positive quality control (PC1) includes four types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments.
（2) Positive quality control (PC2) includes four types of pathogenic bacterial nucleic acids or plasmid DNA containing specific fragments.
【 Precautions 】
Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.
1. Suggest partitioning operation
（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.
（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.
（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.
