SBA-78 Biosensing Analyzer

SBA-78 Biosensing Analyzer

Section One working principle

SBA-70The type of biosensing automatic analyzer uses enzymatic reactions for quantitative analysis, and the key sensors for measurement are immobilized enzymes andH₂O₂The electrode composite sensor is analyzed based on the following biochemical reactions:

Accurately diluted with buffer solution (ensuringpHin7A sample with a certain ion strength (left and right) is quantitatively aspirated by an automatic sampling needle and then pushed to the surface of the immobilized enzyme sensor by a flow pump. The substrate in the sample reacts with the immobilized enzyme membrane, and the reaction releasesH₂O₂Through the inner layer of the enzyme membrane, electrons are lost on the surface of the platinum electrode and reduced toH₂OThe platinum electrode obtains electrons to generate a current signal, which is related toH₂O₂The concentration is proportional, andH₂O₂The concentration is linearly proportional to the substrate concentration. By comparing the measured substance with the standard sampleH₂O₂Quantity can be used to calculate the content of substrate in the tested sample. After the reaction is completed, the sample is pushed into the waste liquid bottle by the flow pump. When the residual sample on the surface of the immobilized enzyme sensor is lower than the concentration that does not affect the next measurement, the next sample measurement will automatically start.

Section Two Characteristics of Biosensing Analyzer

（1）The reaction is catalyzed by immobilized enzymes, and expensive biochemical reagents can be reused multiple times. A single enzyme membrane can be measured thousands of times, so the total measurement cost is relatively low. (Table 1)

Easy to operate: The sample and standard solution are processed in1.5mlDiluted in centrifuge tube(Actual installation1.000ml)Place it in the sample tray, simply press the measurement button, and the instrument will automatically complete all operations including calibration, linear correction, and sample measurement, until all samples on the sample tray are measured without manual intervention. Each sample can be automatically measured on site after completion(Or afterwards)Print out the results(Including measurement number, time, and analysis value), measurement results

（1）It is also saved in the instrument memory and can be viewed and printed at any time. The required amount of sample before dilution for measurement is20Micro liter.

Table 1:SBA-70Cost estimation of reagent kit for analyzer measurement (unit: yuan)

*According to the lactate enzyme membrane100yuan/One, glucose enzyme membrane80yuan/One, big bag buffer solution40yuan/5000ml,standard solution35yuan/100ml，The working life of enzyme membrane is determined by1Monthly calculation. Daily measurement100Secondly, the consumption cost of the reagent kit is lower than the consumption of disposable centrifuge tubes for preparing samples (approximately per tube)0.10Yuan). For samples that do not involve hygiene issues, such as fermented foods, disposable centrifuge tubes can also be washed and reused to further reduce costs.

（2）Fast analysis speed: The measurement cycle of each sample, including injection, reaction, and cleaning, is insufficient in all operations1Minutes, actual sample volume can be measured per hour60~100One.

（3）The results are accurate, with a general error of less than2%Not affected by color, turbidity, etc.

（4）As a special biochemical instrument, operation and maintenance require a certain amount of biochemical knowledge (such as neutralizing samples with strong acids or alkalis in advance to avoid enzyme membrane deactivation), and the performance of the instrument varies with the service life of the enzyme membrane.

Section Three SBA-70Performance indicators of type analyzer

SBA-70The type analyzer adopts LCD full Chinese display, and all operations can be realized through the Chinese menu, which is intuitive and clear. After completing the pre dilution of the sample, place the measured sample into the sample tray and simply press one button to complete the measurement of all samples. It can be set to automatically print the results after each sample measurement is completed. All instrument settings can also be operated throughPCMachine implementation, instruments andPCThe machine is connected through a serial port, and after connecting, it can not only set the parameters of the instrument, but also read and delete saved data. read inPCThe data is saved in a dedicated format and can be annotated and queried.

SBA-70The main performance of the analyzer is as follows:

Table 2: SBA-70Performance indicators of type analyzer

SBA-78 Biosensing Analyzer