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No. 19 Keyuan Road, Lixia District, Jinan City
Shandong Baisentaike Instrument Co., Ltd
No. 19 Keyuan Road, Lixia District, Jinan City
SBA-70The type of biosensing automatic analyzer uses enzymatic reactions for quantitative analysis, and the key sensors for measurement are immobilized enzymes andH2O2The electrode composite sensor is analyzed based on the following biochemical reactions:
bottom thing |
Immobilized enzyme membrane |
production thing |
||
amino acid |
O2+H2O |
Amino acid oxidase |
alpha-Ketoglutaric acid+NH4+ |
H2O2 |
glucose |
O2+H2O |
glucose oxidase |
Gluconic acid |
H2O2 |
lactic acid |
O2+H2O |
Lactate oxidase |
Pyruvic acid |
H2O2 |
ethanol |
O2+H2O |
Ethanol oxidase |
acetic acid |
H2O2 |
Accurately diluted with buffer solution (ensuringpHin7A sample with a certain ion strength (left and right) is quantitatively aspirated by an automatic sampling needle and then pushed to the surface of the immobilized enzyme sensor by a flow pump. The substrate in the sample reacts with the immobilized enzyme membrane, and the reaction releasesH2O2Through the inner layer of the enzyme membrane, electrons are lost on the surface of the platinum electrode and reduced toH2OThe platinum electrode obtains electrons to generate a current signal, which is related toH2O2The concentration is proportional, andH2O2The concentration is linearly proportional to the substrate concentration. By comparing the measured substance with the standard sampleH2O2Quantity can be used to calculate the content of substrate in the tested sample. After the reaction is completed, the sample is pushed into the waste liquid bottle by the flow pump. When the residual sample on the surface of the immobilized enzyme sensor is lower than the concentration that does not affect the next measurement, the measurement of the next sample will automatically start