Rubella Antigen (BR2S)

📝 Product basic information

Item number: EL-05-15

Product NameRubella Antigen (BR2S)(BR2S type)

Type: Recombinant rubella virus structural protein antigen (mainly E1 glycoprotein)

Purpose: Used for the research and production of rubella virus detection kits (such as ELISA, CLIA, immunochromatography, etc.), as coating/labeling antigens.

Specifications: Provides various packaging options such as 100 μ g, 500 μ g, 1mg, etc. (can be customized according to requirements); The standard concentration is 1mg/ml (in liquid form, containing a stabilizing protective agent).

Storage conditions: Short term storage at 2-8 ℃ (1 month); Long term recommendation: -20 ℃ (avoid repeated freezing and thawing).

Validity period: 24 months from the date of production (unopened).

🔍 Detailed Introduction: The Scientific Path from "Virus Targets" to "Detection Tools"

Microbix's Rubella Antigen is a highly specific recombinant antigen targeting the Rubella virus. The core technology is derived from the company's in-depth analysis of the Rubella virus genome. The E1 glycoprotein coding sequence with immunogenicity on the virus surface is screened and expressed using the insect cell expression system (Sf9 cells) for recombinant expression. After multi-step affinity chromatography purification, the antigen is produced.

Key features:

-Target accuracy: Focus on the rubella virus E1 glycoprotein (the main vector of virus invasion into the host and also the main target for the production of neutralizing antibodies in the human body) to ensure the specificity of detection;

-Reliable process: using serum-free culture medium and affinity chromatography purification to avoid contamination by impurities, antigen purity verified by SDS-PAGE>95%;

-Stable activity: Through in vitro neutralization tests, it has been verified that the antigen can efficiently bind to polyclonal/monoclonal antibodies (binding activity ≥ 85%), and the activity decreases by less than 5% after long-term storage (under -20 ℃ conditions).

💎 Core advantage: Why choose BR2S rubella antigen?

one ️⃣ High compatibility: Supports multiple detection platforms such as ELISA, CLIA, and immunochromatography, and is compatible with mainstream packaging buffers (such as PBS, CBS) and markers (enzymes, fluorescein, colloidal gold);

two ️⃣ Low background interference: After lipid and nucleic acid removal treatment, the non-specific binding rate is less than 3% (conventional raw materials generally exceed 8%), significantly reducing the risk of false positives;

three ️⃣ Compliance assurance: Production complies with ISO 13485 medical device quality management system certification, and raw materials can be traced back to WHO rubella virus reference materials (NIBSC code: VR-1066);

four ️⃣ Technical support: Provide free sample trials, suggestions for optimizing testing plans, and batch consistency reports (CV<5%).

❓ Frequently Asked Questions (Q&A)

Q1: What types of detection kits are suitable for BR2S antigen?

A: The ELISA kit is widely used for qualitative/quantitative detection of rubella virus IgM/IgG antibodies, and can also be used for colloidal gold rapid detection test strips (with optimized concentration of corresponding markers).

Q2: Are there any special requirements for the storage conditions of antigens? What should be noted during transportation?

A: Long term storage recommendation -20 ℃ (avoid repeated freezing and thawing); Short term (within 1 month) can be stored at 2-8 ℃. During transportation, foam box+ice bag shall be used to ensure the temperature ≤ 25 ℃ (delivery within 48 hours will not affect the activity).

Q3: Can Microbix support customizing antigens of different concentrations or modifications?

A: Okay! We provide customized services, including concentration adjustment, coupling modification (fluorescein, etc.), and optimization of special buffer formulations. Prior communication of requirements and signing of agreements are required.

Q4: What are the advantages of BR2S antigen compared to natural rubella virus antigen?

A: Natural antigens have issues such as incomplete virus inactivation, large inter batch differences, and potential biosafety risks; BR2S is a recombinant antigen produced through genetic engineering technology, with no live virus contamination and high inter batch consistency (CV<5%), making it safer and more controllable.

Q5: How to verify the performance of the detection kit using BR2S antigen?

A: Suggest evaluating through the following indicators:

-Sensitivity: The consistency rate with clinically positive samples is ≥ 95%;

-Specificity: No cross reactivity with rubella virus negative samples or other viruses (such as measles and Toxoplasma gondii);

-Stability: Activity retention ≥ 80% after accelerated testing at 37 ℃ for 7 days.

Q6: What file support does Microbix provide?

A: Each batch of products comes with a COA (Certificate of Analysis), which includes key indicators such as purity (SDS-PAGE), activity (neutralization test data), endotoxin (≤ 1EU/μ g), and host protein residue (<100ppm); If ISO 13485 system certification documents are required, they can be provided separately.

Q7: What is the minimum order quantity? How long is the delivery cycle?

A: The minimum order quantity for regular packaging is 100 μ g, and the delivery cycle is 7-10 working days (when there is sufficient stock in stock); Customized requirements need to be communicated 15 days in advance.

Q8: Will the activity of antigens decrease with storage time? How to determine if it has failed?

A: If the activity decay is less than 5% within 24 months of storage at -20 ℃, it is recommended to conduct ELISA pre experiment verification before use (OD value should be ≥ 0.8 when reacting with known positive antibodies). If the OD value significantly decreases (<0.5), it may have failed.

Q9: Has the BR2S antigen been internationally certified (such as FDA, CE)?

A: At present, raw material grade products have not directly applied for FDA/CE, but their production complies with ISO 13485 standards and has been verified by multiple IVD companies to be suitable for the development of CE certified reagent kits.

For more product information, please contact our regional agent in China: Shanghai Qifa Experimental Reagent Co., Ltd

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(Note: The content of this article is based on brand public information and industry standard information, and the specific information is subject to the brand information document.)

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