Red blood cell lysate

1、 Product Introduction

Baiao YikangRed blood cell lysate

Red Blood Cell Lysis Solution(Item number:BA3311）

High throughput single-cell sequencing requires high quality single-cell suspensions. In addition to parameters such as cell viability, the nucleation rate of single-cell suspensions is an important factor affecting the quality of subsequent data. A lower nucleation rate may lead to inaccurate identification of effective cells, blurred boundaries between effective cells and non cells, high or low number of captured cells, background elevation, and other adverse phenomena. Excessive red blood cell content is one of the important reasons for low nuclear rate.

The Baiao Yikang team has accumulated10,000+Experience in single-cell sequencing services for individual samples, involving150+Various types of organizations with rich experience in fresh tissue dissociation. On this basis, Baiaoyikang independently developedRed blood cell lysateBy using this lysis buffer, red blood cells in single-cell suspension can be efficiently lysed, improving the quality of the suspension.

2、 Product advantages

Wide applicability:Rapid and efficient lysis of red blood cells in mammalian cell suspensions.

Good performance:It can quickly and efficiently lyse red blood cells in single-cell suspension, while almost not harming other lymphocytes or nucleated cells, greatly improving the quality of single-cell suspension and obtaining higher quality data results.

Multiple application scenarios:Suitable for optimizing cell suspensions containing a large number of red blood cells, and can be applied to high-throughput single-cell sequencing and other related scientific research experiments.

IIItest effect

We appreciate fresh peoplePBMCExperiments were conducted, and it can be seen from the cell diameter distribution map that after red blood cell lysis treatment, the distribution of red blood cells was significantly reduced, and the nucleation rate parameter of single-cell suspension also decreased32.4%upgrade to92.6%And the activity has also been significantly improved. From the data analysis results, it can be seen that after red blood cell lysis treatment, the median measured genes significantly improved497upgrade to1,666.

Testing process:

Test results:

Beijing Baiao Innovation Technology Co., Ltd. welcomes inquiries and quotations.