Mouse CD3 T Cell Isolation Kit

NegotiableUpdate on 05/06

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Overview

Beijing Baiao Innovation Technology Co., Ltd. provides the immuno-X mouse CD3 T cell isolation kit. For more information about immuno-X products, please feel free to inquire!

Product Details

1Product Introduction

The mouse CD3 T cell isolation kit uses a negative selection method to isolate the mouse spleen through a column free magnetic poleCD3 Tcell During the separation process, antibodies and magnetic beads will not come into contactCD3 TCells that can maintain maximum stabilityTThe most primitive state of cells.
•Easy to operate
•No need to crack red
•The highest purity can reach99%
• 18-20Complete separation in minutes
The reagent kit is labeled with antibodies conjugated with biotinCD3 TCells are incubated and coupled with streptavidin nanomagnetic beads, followed by column free separation using magnetic poles. The target cells can be separated by simply pouring them into a new tube, and the separated cells can be immediately used for subsequent applications such as flow cytometry, cell culture, and functional experiments.

IIKit components

Product Name	Storage conditions	Composition of buffer solution	function
EasyIso™Mouse CD3 T cell Isolation Cocktail	4°CSave and avoid freezing	PBS, 0.1% BSA	Identify nonCD3 TCell antibody mixture, labeling non target cells
EasyIso™Streptavidin Nanobeads	4°CSave and avoid freezing	PBS, 0.1% BSA	Magnetic bead binding nonCD3 TCells, removed by magnetic poles

IIIoperating steps

step	explanation	Dose and time
1	Prepare spleen single-cell suspension to the specified concentration	1 x 10^8 cells/mL
2	joinEasyIso™Mouse CD3 T cell Isolation CocktailTo the sample	100 μL/mL
3	Antibody and cell incubation	Incubation at room temperature10 min
4	Shake and mix wellStreptavidin Nanobeads	mix well30 s
5	plusStreptavidin NanobeadsTo the sample	100 μL/mL
6	Gently mix magnetic beads and cells, incubate	Incubation at room temperature5 min
7	joinFACS BufferGently mix into the sample	Total volume added to2.5 mL（5 mLFlow tube) Total volume added to7.5 mL (15 mLcentrifuge tube)
8	Place the sample on a magnetic rack to allow the magnetic beads to adsorb	Single hole magnetic frame adsorption（3 min） Joint row magnetic frame adsorption（5 min）
9	Tilt the magnetic frame and pour the sample into a new collection tube to collect the sample	Cell separation successful

More Inno-XMouse CD3 T Cell Isolation KitWelcome to inquire about Imuno-X reagent kit agent Beijing Baiao Innovation Technology Co., Ltd!

小鼠CD3 T细胞分离试剂盒