- Phone
-
Address
Community supporting commercial building in Chuangke Town, Wenquan Town, Haidian District, Beijing
Beijing Baiao Innovation Technology Co., Ltd
Mouse DN T Cell Isolation Kit
NegotiableUpdate on 05/06
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
Beijing Baiao Innovation Technology Co., Ltd. provides the immuno-X mouse DN T cell isolation kit. For more information about immuno-X products, please feel free to inquire!
Product Details
1Product Introduction
The immuno-X mouse DN T cell isolation kit uses a negative selection method to isolate mouse spleen cells using a column free magnetic poleCD4-CD8-Double negative（Double Negative, DNofCD3 Tcell During the separation process, antibodies and magnetic beads will not come into contactDN CD3 TCells that can maintain maximum stabilityTThe most primitive state of cells.
●Easy to operate
●No need to crack red
●The highest purity can reach99%
●18-20Complete separation in minutes
The reagent kit is labeled with non biological SU conjugated antibodiesDN CD3 TCells are incubated and coupled with streptavidin nanomagnetic beads, followed by column free separation using magnetic poles. The target cells can be separated by simply pouring them into a new tube, and the separated cells can be immediately used for subsequent applications such as flow cytometry, cell culture, and functional experiments.
IIKit components
Product Name |
Storage conditions |
Composition of buffer solution |
function |
EasyIsoTMMouse DN CD3 TCell Isolation Cocktail |
4°CSave and avoid freezing |
PBS, 0.1% BSA |
Identify nonDN CD3 Tcell |
EasyIsoTMStreptavidin Nanobeads |
4°CSave and avoid freezing |
PBS, 0.1% BSA |
Magnetic bead binding nonDN CD3 TCells, removed by magnetic poles |
IIIoperating steps
step |
explanation |
Dose and time |
Prepare spleen single-cell suspension to the specified concentration |
1 x 10^8 cells/mL |
|
2 |
joinEasyIsoTMMouse DN CD3 T cell Isolation CocktailTo the sample |
100 ML/mL |
3 |
Antibody and cell incubation |
Incubation at room temperature10 min |
4 |
Shake and mix wellStreptavidin Nanobeads |
mix well30 s |
5 |
plusStreptavidin NanobeadsTo the sample |
100 ML/mL |
6 |
Gently mix magnetic beads and cells, incubate |
Incubation at room temperature5 min |
joinFACS BufferGently mix into the sample |
Total volume added to2.5 mL（5 mLFlow tube) Total volume added to 7.5 mL (15 mLcentrifuge tube) |
|
8 |
Place the sample on a magnetic rack to allow the magnetic beads to adsorb |
Single hole magnetic frame adsorption（3 min） Joint row magnetic frame adsorption（5 min） |
9 |
Tilt the magnetic frame and pour the sample into a new collection tube to collect the sample |
Cell separation successful |
More about Inno-XMouse DN T Cell Isolation KitWelcome to consult Beijing Baiao Innovation Technology Co., Ltd. for our introduction!
