Recombinant human platelet-derived growth factor BB(rhPDGF-BB) Product Technical Manual

1. Product Overview

Recombinant human platelet-derived growth factor BB（Recombinant Human Platelet-Derived Growth Factor-BB, rhPDGF-BB）It is a homodimeric protein belonging to the PDGF family, which has good biological activity and wide applications. Natural PDGF exists in various forms such as AA, AB, BB, CC, DD, etcPDGF-BBDue to its high affinity binding to all types of PDGF receptors (alpha alpha, alpha beta, beta beta), it is known as a "universal ligand".

This product utilizes genetic recombination technology (usuallyE. coliThe preparation of the expression system is a homodimer connected by disulfide bonds, with a molecular weight of approximately 24.3 kDa. The rhPDGF-BB we provide has high purity, high specific activity, and low endotoxin properties, making it suitable for various scenarios ranging from basic cell biology research to preclinical drug development.

2. Mechanism of Action

RhPDGF-BB is a potentMitogen, a mitogenIts mechanism of action mainly involves binding and activating tyrosine kinase receptors (PDGFRs) on the surface of the cell membrane:

1. Receptor binding and activation:RhPDGF-BB can simultaneously bind to PDGFR - α and PDGFR - β, inducing receptor dimerization and autophosphorylation.

2. Signal cascade conduction:Activate multiple downstream signaling pathways including Ras MAPK, PI3K-AKT, and PLC - γ.

3. Biological effects:

• Cell proliferation:Strongly stimulate the division and proliferation of fibroblasts, smooth muscle cells (SMCs), glial cells, and mesenchymal stem cells (MSCs).

• Cell chemotaxis:Inducing the directed migration of neutrophils, monocytes, and fibroblasts towards the damaged site, initiating the repair phase after the inflammatory response.

• Matrix synthesis:Promote the synthesis of collagen, fibronectin, and hyaluronic acid, and accelerate the formation of granulation tissue.

3. Key Features of the Product

• Biological activity:Each batch has been validated through mouse BALB/c 3T3 cell proliferation experiments. ED50 is usually used in1 - 5 ng/mLWithin the range, the specific activity should be ≥ 2.0 × 10 ^ 5 IU/mg, ensuring significant effects can be achieved with minimal addition.

• Natural dimer structure:Adopting optimized refolding technology to ensure the formation of correct disulfide linked products is a necessary condition for maintaining the biological function of PDGF-BB (monomers are almost inactive).

• High purity and safety:Purity>95% (typical value>98%) was detected by SDS-PAGE and HPLC. The endotoxin level is less than 0.1 EU/μ g (LAL method), with no risk of pathogen contamination, suitable for sensitive primary cell and stem cell culture.

• Batch stability:A strict GMP level quality management system ensures that the activity difference between different batches (Lot to Lot) is controlled within 5%, ensuring the reproducibility of experimental data.

4. Key application areas (Applications)

A. Wound Healing and Regenerative Medicine

• Research on Difficult to Treat Ulcers:Simulate the action mechanism of becapramine, and use it in the in vitro model or animal experiment of diabetes foot ulcer (DFU), pressure sore and venous ulcer to study its efficiency in promoting granulation tissue growth and epithelial regeneration.

• Bone repair:RhPDGF-BB is often combined with bone repair materials to accelerate fracture healing and periodontal tissue regeneration by chemotactic bone precursor cells and promoting angiogenesis.

B. Stem Cell Culture

• MSC amplification:RhPDGF-BB is a key additive in the in vitro expansion medium of mesenchymal stem cells (MSCs). It can not only significantly increase the proliferation rate of MSCs, but also maintain their multipotent differentiation potential (Stemness) and reduce cell aging.

C. Cardiovascular and Smooth Muscle Research

• Vascular remodeling:To study the pathophysiological mechanism of PDGF-BB in atherosclerosis, vascular smooth muscle cell migration and vascular wall remodeling.

• Vascular stability:In tissue engineering, PDGF-BB helps recruit pericytes and promote the maturation and stability of neovascularization.

5. Quality Commitment

Every rhPDGF-BB product undergoes strict physical and chemical property analysis and biological functional verification before leaving the factory. We promise to provide customers with traceable quality inspection reports (COA). Whether you are exploring fundamental mechanisms or conducting translational medicine research, we are your trusted partner.

Note: This product is for research use only and cannot be directly used for human injection or sold as a drug.