Recombinant human epidermal growth factor(rhEGF) Product Technical Manual

1. Product Overview

Recombinant human epidermal growth factor（Recombinant Human Epidermal Growth Factor, rhEGF）It is a bioactive peptide produced through genetic recombination technology. It consists of 53 amino acid residues with a molecular weight of approximately 6.2 kDa, and its amino acid sequence is consistent with the naturally occurring epidermal growth factor in the human body.

As a potent cell division factor, rhEGF is known as the "beauty factor" and "repair expert" in the fields of biomedicine, skincare, and life sciences research. This product utilizes Escherichia coli（E. coli）The preparation of the secretion expression system, after multi-step chromatography purification, ensures the high purity, high specific activity, and low endotoxin level of the product, meeting the high standard requirements of scientific research and raw material drug grade.

2. Mechanism of action

The core mechanism by which rhEGF acts is its specific binding to the epidermal growth factor receptor (EGFR) on the cell membrane surface

1. Receptor binding:RhEGF binds to EGFR on the target cell membrane, inducing receptor dimerization.

2. Signal transduction:Dimerization activates the intracellular tyrosine kinase activity of receptors, initiating downstream signaling cascades such as RAS-MAPK and PI3K-AKT pathways.

3. Biological effects:The signal is transmitted to the nucleus, promoting the synthesis of DNA, RNA, and proteins, thereby accelerating cell mitosis and promoting the proliferation and migration of epithelial cells and fibroblasts. In addition, it can stimulate the synthesis of extracellular matrix such as hyaluronic acid and glycoproteins.

3. Core advantages of the product

The rhEGF products we provide have the following significant technical advantages:

• High biological activity:Verified by mouse BALB/c 3T3 cell proliferation experiments, the specific activity is ≥ 1.0 × 10 ^ 7 IU/mg. Micro addition can produce significant cell proliferation effects.

• High purity guarantee:SDS-PAGE detection showed purity greater than 95%. The content of impurities is extremely low, effectively reducing non-specific reactions.

• Strict security:No animal free ingredients, eliminating the risk of cross infection between prions and animal viruses. The endotoxin level is extremely low (<0.1 EU/μ g), suitable for safety requirementstallCell culture and formulation development.

• Excellent stability:The activity retention time is long, and the lot to lot variation is less than 5%.

4. Wide range of application areas

A. Medical Repair and Wound Healing (Medical Application)

RhEGF is widely used in clinical practice to promote the healing of various types of wounds:

• Burn and scald treatment:Accelerate epidermal coverage, shorten healing time, and reduce scar formation.

• Chronic ulcer:It has a significant effect on diabetes foot ulcer, bedsore and varicose ulcer.

• Ophthalmic applications:Used for post corneal transplant repair and healing of corneal injuries.

B. Medical Skincare (Cosmetic Application)

In functional skincare products, rhEGF is the core ingredient for anti-aging and repair:

• Postoperative repair:Suitable for repairing damaged barriers after medical beauty treatments such as laser, microneedle, and fruit acid peels, relieving redness and swelling, and accelerating scab shedding.

• Anti wrinkle and firming:Promote the synthesis of collagen and elastin, improve skin elasticity, and smooth fine lines.

• Acne removal and leveling:By accelerating the renewal of epidermal cells, effectively preventing the formation of acne scars and fading acne scars.

C. Life Science Research (Research Application)

As a key growth factor, it is an essential additive in serum-free cell culture media:

• Used for maintaining and expanding stem cells such as iPSCs and MSCs.

• Used to construct models of organoids.

• Used to study the signal transduction pathways of tumor cells.

5. Technical Specifications

6. Quality Commitment

Each batch of products undergoes strict physical and chemical property testing and biological activity verification before leaving the factory, and detailed quality inspection reports (COA) are provided. We are committed to providing customers with high-quality raw materials with high cost-effectiveness, high activity, and high purity, to help you achieve success in research and production.