Recombinant human bone morphogenetic protein-4(rhBMP-4) Product Technical Manual

1. Product Overview

Recombinant human bone morphogenetic protein-4（Recombinant Human Bone Morphogenetic Protein-4, rhBMP-4）It is a multifunctional secreted signaling protein belonging to the transforming growth factor - β (TGF - β) superfamily. It is a key factor in the process of embryonic development, not only dominating the formation of bones and cartilage, but also playing a central role in the formation of the embryonic dorsal ventral axis, organogenesis, and the fate determination of pluripotent stem cells.

This product is produced through genetic recombination technology（ E. coliHigh purity protein prepared by expression system. After strict refolding and chromatographic purification processes, the rhBMP-4 we provide has a natural spatial folding structure and biological activity, and is widely used in the maintenance and differentiation of pluripotent stem cells (iPSCs/ESCs), tissue engineering, and regenerative medicine research.

2. Mechanism of Action

RhBMP-4 initiates signal transduction by binding to serine/threonine kinase receptors on the surface of target cells, and its core mechanism includes:

1. Formation of receptor complexes:The rhBMP-4 dimer first binds to type I receptors (such as ALK-3/6) and type II receptors (such as BMPR-II) to form a heterologous tetramer complex.

2. Activation of Smad pathway:Receptor complexes phosphorylate intracellular effector molecules Smad 1/5/8. Phosphorylated Smad 1/5/8 binds to Smad 4 and is transferred to the nucleus.

3. Gene transcription regulation:After entering the nucleus, Smad complexes act as transcription factors to regulate the expression of specific target genes (such as Runx2, Osterix, etc.), thereby inducing osteogenic differentiation or altering the differentiation direction of stem cells.

3. Key Features of the Product

• High biological activity:The activity of the product was validated by inducing alkaline phosphatase (ALP) production in ATDC5 cells (mouse teratoma cells). ED50 is usually used in5 - 20 ng/mLBetween them, it exhibits osteogenic induction ability.

• High purity and uniformity:According to SDS-PAGE analysis, the purity is>95% (or even>98%).

• Stem cell grade quality standards:Strictly control the endotoxin content (<0.1 EU/μ g) and conduct sterile and mycoplasma testing to ensure no contamination and non toxicity during long-term stem cell culture, especially suitable for sensitive embryonic stem cell research.

• No animal derived ingredients (optional):We can provide rhBMP-4 produced in serum-free and animal free culture systems to eliminate potential pathogen risks and meet preclinical research needs.

4. Key application areas (Applications)

RhBMP-4 is one of the widely used cytokines in life science research:

A. Stem Cell ResearchCore application

• Mesoderm Induction:RhBMP-4 is the gold standard factor for inducing differentiation of human pluripotent stem cells (hESCs/hiPSCs) into the mesoderm, used to generate cardiomyocytes, hematopoietic stem/progenitor cells, and endothelial cells.

• Trophoblast differentiation:Under specific conditions, BMP-4 can induce differentiation of hESCs into Trophoblasts.

• Maintaining pluripotency (mice):In the culture of mouse embryonic stem cells (mESCs), BMP-4 is often combined with LIF (leukemia inhibitory factor) to replace serum or feeder layer and maintain the undifferentiated state of cells (Ground State).

B. Organizational Engineering and Regenerative Medicine

• Bone and cartilage repair:As a potent osteogenic inducer, rhBMP-4 is widely incorporated into biological scaffold materials such as collagen and hydroxyapatite for accelerating bone defect repair and bone fusion research.

• Dental regeneration:Study the differentiation of periodontal ligament cells and the regeneration of alveolar bone.

C. Developmental Biology

• Study the early embryonic development of dorsal ventral axis fixation, limb bud formation, and developmental inhibition mechanisms of the nervous system.