Rat Kidney Injury Molecule-1 (Kim-1) ELISA Detection Kit

1、 Product information:

Product Name:Rat Kidney Injury Molecule-1 (Kim-1) ELISA Detection Kit

Product Code: YXR31104

Product batch number: see product label for details

Validity period: 6 months

Storage conditions: 2-8 ℃

Product Usage

This kit is used for in vitro quantitative detection of the content of rat kidney injury molecule 1 (Kim-1) in serum, plasma, tissue homogenate, and related liquid samples.

2、 Detection principle:

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores of the rat kidney injury molecule 1 (Kim-1) capture antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of rat kidney injury molecule 1 (Kim-1) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

3、 Sample collection, processing, and preservation methods:

The following is just a general guide for sample collection. Sodium azide should not be used as a preservative during all sample collection processes.

1. Serum: Use test tubes that do not contain pyrogens or endotoxins, and avoid any cell irritation during the operation. Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or overnight at 4 ℃, then centrifuge at 1000 × g for 20 minutes, and take the supernatant. Alternatively, store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

2. Plasma: Collect specimens using EDTA or heparin as anticoagulants, and centrifuge the specimens at 2-8 ℃ 1000 × g for 15 minutes within 30 minutes after collection. The supernatant can be collected for detection, or stored at -20 ℃ or -80 ℃, but repeated freezing and thawing should be avoided.

3. Cell culture supernatant or other biological specimens: Centrifuge at 1000 × g for 20 minutes to obtain the supernatant for detection, or store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

4. Tissue homogenate: Rinse the tissue with pre cooled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate can affect measurement results), weigh and cut the tissue into pieces. Mix the shredded tissue with the corresponding volume of PBS (usually in a weight to volume ratio of 1:9, for example, 1g of tissue sample corresponds to 9mL of PBS, the specific volume can be adjusted appropriately according to experimental needs, and records should be kept. Recommend adding protease inhibitors to PBS and grinding thoroughly on ice in a glass homogenizer. To further lyse tissue cells, the homogenate can be sonicated or subjected to repeated freeze-thaw cycles. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes and collect the supernatant for detection.

Note: Hemolysis of specimens can affect the final test results, therefore hemolytic specimens are not suitable for this test.

5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

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Rat Kidney Injury Molecule-1 (Kim-1) ELISA Detection Kit