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Shanghai Qifa Experimental Reagent Co., Ltd

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    Shanghai Qifa Experimental Reagent Co., Ltd., No. 6619 Chuansha Road, Chuansha New Town, Pudong New Area, Shanghai (Chuansha Rd # 6619, Pudong, Shanghai, Zipecode: 21200, China)

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Rapid GolgiStain™ Kit Gorky staining kit

NegotiableUpdate on 05/06
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Overview

PK401 Rapid GolgiStain The Kit Golgi Staining Kit is a classic upgraded Golgi staining kit launched by FD Neurotech, designed specifically to clearly present the three-dimensional structure of complete neurons and glial cells in the central nervous system. Based on a century old silver plating principle optimization, this reagent kit forms high contrast metal deposits in neural tissue through a dual immersion plating process (potassium dichromate+mercury salt post-treatment).

Product Details

Product Introduction

PK401 Rapid GolgiStain ™ The Kit Golgi Staining Kit is a classic upgraded Golgi staining kit launched by FD Neurotech, designed specifically to clearly present the three-dimensional structure of complete neurons and glial cells in the central nervous system. Based on a century old silver plating principle optimization, this reagent kit uses a dual immersion plating process (potassium dichromate+mercury salt post-treatment) to form high contrast metal deposition in neural tissue, achieving:

-Applicable specimens: rodent brain tissue (mice/rats), primate brain slices, frozen/paraffin embedded samples

-Thickness compatibility: Supports staining of 100-500 μ m thick sections (recommended 300 μ m)

-Dyeing cycle: Laboratory standard process only takes 5-7 days (traditional scheme takes 2-12 weeks)

-Resolution: Clear display of dendritic spines, axonal branches, and other microstructures ≤ 0.5 μ m

-Output format: compatible with bright field microscopy, confocal 3D reconstruction, and neuromorphometric analysis (such as Neurolucida)


core advantage

✅ Fast acting and stability coexist

The chemical catalytic system improves the immersion efficiency by 300%, and the difference in dyeing depth between batches is ≤ 5% (ISO 13485 certified production line)

✅ Global neuron capture

Breakthrough in overcoming the problem of superficial neuronal overgrowth, achieving clear and synchronized visualization of cortical V-VI pyramidal cells and hippocampal CA1 region

✅ No dependence on genetically modified organisms

No need for fluorescent protein labeling, avoiding limitations of virus transfection or transgenic animal models, especially suitable for pathological models such as Alzheimer's disease

✅ Laboratory friendly type

Ready to use reagent packaging (including A/B/C solutions), operated in the dark throughout the process, including waste liquid treatment guidelines and MSDS safety certification


Purchase common questions and answers

❓ Q1:Rapid GolgiStain ™ Is the Kit Gorky staining kit compatible with tissue after perfusion fixation?

Support cardiac perfusion with 4% paraformaldehyde fixation, but ensure perfusion pressure ≤ 120mmHg to avoid vascular rupture affecting staining uniformity

❓ Q2: How to store stained sections for a long time?

Recommended DPX sealed tablets for storage, can maintain no fading for ≥ 5 years under dark conditions (accelerated aging test data)

❓ Q3: Minimum order quantity and transportation temperature?

Single set minimum order (including 50 pieces of dyeing capacity), cold chain transportation (2-8 ℃), dry ice express delivery supported within China (freight collect)

❓ Q4: How to verify the success rate of staining?

Provide a reference atlas for staining of Purkinje cells in the cerebellum of standard mice. It is recommended to use coronal striated body sections as a positive control for first-time use

❓ Q5: Does it support pathological samples?

Verified by the AD model (APP/PS1 mice), it can clearly show dendritic damage around beta amyloid plaques, but the immersion period needs to be extended to 9 days


For more product information, please contact our regional agent in China: Shanghai Qifa Experimental Reagent Co., Ltd

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(Note: The content of this article is based on brand public information and industry standard information, and the specific information is subject to the brand information document.)


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