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Shanghai Qifa Experimental Reagent Co., Ltd., No. 6619 Chuansha Road, Chuansha New Town, Pudong New Area, Shanghai (Chuansha Rd # 6619, Pudong, Shanghai, Zipecode: 21200, China)
Shanghai Qifa Experimental Reagent Co., Ltd
Shanghai Qifa Experimental Reagent Co., Ltd., No. 6619 Chuansha Road, Chuansha New Town, Pudong New Area, Shanghai (Chuansha Rd # 6619, Pudong, Shanghai, Zipecode: 21200, China)
✨Product Introduction
PK401 Rapid GolgiStain ™ The Kit Golgi Staining Kit is a classic upgraded Golgi staining kit launched by FD Neurotech, designed specifically to clearly present the three-dimensional structure of complete neurons and glial cells in the central nervous system. Based on a century old silver plating principle optimization, this reagent kit uses a dual immersion plating process (potassium dichromate+mercury salt post-treatment) to form high contrast metal deposition in neural tissue, achieving:
-Applicable specimens: rodent brain tissue (mice/rats), primate brain slices, frozen/paraffin embedded samples
-Thickness compatibility: Supports staining of 100-500 μ m thick sections (recommended 300 μ m)
-Dyeing cycle: Laboratory standard process only takes 5-7 days (traditional scheme takes 2-12 weeks)
-Resolution: Clear display of dendritic spines, axonal branches, and other microstructures ≤ 0.5 μ m
-Output format: compatible with bright field microscopy, confocal 3D reconstruction, and neuromorphometric analysis (such as Neurolucida)
✨core advantage
✅ Fast acting and stability coexist
The chemical catalytic system improves the immersion efficiency by 300%, and the difference in dyeing depth between batches is ≤ 5% (ISO 13485 certified production line)
✅ Global neuron capture
Breakthrough in overcoming the problem of superficial neuronal overgrowth, achieving clear and synchronized visualization of cortical V-VI pyramidal cells and hippocampal CA1 region
✅ No dependence on genetically modified organisms
No need for fluorescent protein labeling, avoiding limitations of virus transfection or transgenic animal models, especially suitable for pathological models such as Alzheimer's disease
✅ Laboratory friendly type
Ready to use reagent packaging (including A/B/C solutions), operated in the dark throughout the process, including waste liquid treatment guidelines and MSDS safety certification
✨Purchase common questions and answers
❓ Q1:Rapid GolgiStain ™ Is the Kit Gorky staining kit compatible with tissue after perfusion fixation?
Support cardiac perfusion with 4% paraformaldehyde fixation, but ensure perfusion pressure ≤ 120mmHg to avoid vascular rupture affecting staining uniformity
❓ Q2: How to store stained sections for a long time?
Recommended DPX sealed tablets for storage, can maintain no fading for ≥ 5 years under dark conditions (accelerated aging test data)
❓ Q3: Minimum order quantity and transportation temperature?
Single set minimum order (including 50 pieces of dyeing capacity), cold chain transportation (2-8 ℃), dry ice express delivery supported within China (freight collect)
❓ Q4: How to verify the success rate of staining?
Provide a reference atlas for staining of Purkinje cells in the cerebellum of standard mice. It is recommended to use coronal striated body sections as a positive control for first-time use
❓ Q5: Does it support pathological samples?
Verified by the AD model (APP/PS1 mice), it can clearly show dendritic damage around beta amyloid plaques, but the immersion period needs to be extended to 9 days
For more product information, please contact our regional agent in China: Shanghai Qifa Experimental Reagent Co., Ltd
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(Note: The content of this article is based on brand public information and industry standard information, and the specific information is subject to the brand information document.)
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