Photochemical derivatizer

The photochemical derivatization method is a method based on the absorption of ultraviolet radiation by the analyte in a special photochemical reaction system, which causes changes in the properties or structure of the substance, resulting in fluorescence enhancement, and changes in the fluorescence properties of the analyte to improve the sensitivity of fluorescence analysis.

HX-DVT0010Photochemical derivatizerWidely used in liquid chromatography detection and analysis, it is placed between the chromatography column and the detector for continuous post column photochemical derivatization reactions to improve the sensitivity and selectivity of fluorescence, UV, electrochemical detection, and chemiluminescence detectors.

Has the following significant advantages:

Easy installation, high sensitivity

Photochemical derivatizerWhen in use, place it between the chromatographic column and the detector for continuous post column photochemical derivatization reaction. When using liquid chromatography fluorescence method to detect aflatoxin, the sensitivity of aflatoxin B1 can reach below 0.1 ppb, and the sensitivity of sulfonamide drugs can also reach around 10 ppb.

The experimental operation is simple and easy to control

The addition of photons as derivative reagents is determined by the switching of the ultraviolet light source; Compared to chemical derivatives, there is no need to prepare and store chemical reagents, nor to consider issues such as degradation, shelf life, and disposal of reagents;

Safe operation and cost reduction

Some chemical reagents are toxic, while photochemical derivatives only require controlling the light source switch and do not require contact with toxic reagents. And there is no need to add additional pumps, reactors, heaters, etc., which also minimizes factors that may interfere with the measurement.

Note: In addition to being used for aflatoxin detection, the photochemical column derivatization reaction device can also be applied to the separation of a large number of acid salts, amino acids, peptides, vitamins, sulfonamide drugs, as well as traditional Chinese medicine varieties such as tangerine peel, fatty sea, silkworm, jujube kernel, peach kernel, etc

Product parameters

Product features

• Safety: The instrument casing is a closed shell, and ultraviolet light does not leak out, making it safe and reliable

• Pressure resistance: The derivative pipeline has a pressure resistance of over 2000psi

• Sensitivity: Some substances can reach below 0.1ppb

• Efficient: Response value can be increased by more than 5 times

• Volume: The volume of the reaction tank is about 1.1mL

• Lifespan: The lifespan of ultraviolet lamps can reach over 9000 hours, and it is recommended to control the usage time within 2500 hours (the radiation intensity of ultraviolet lamps will gradually decrease with the extension of usage time); There is a small radiator that can be used for a long time to maintain the temperature inside the enclosed shell at around 50 degrees Celsius, preventing internal components from aging and being damaged due to high temperatures.

• Quick: Equipped with efficient electronic ballasts, it lights up quickly without flicker

Product application:

1. Detection of aflatoxin

AFB1 and AFB2 can produce blue purple fluorescence under ultraviolet light, while AFG1 and AFG2 produce yellow green fluorescence. Therefore, both TLC and HPLC use fluorescence analysis methods. However, when AFB1 and AFG1 come into contact with water, they will undergo fluorescence quenching, resulting in a significant reduction in the fluorescence signal and making it impossible to achieve quantitative detection at the ppb level.