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Nanjing Hexi Biotechnology Co., Ltd
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Online protection pillar

NegotiableUpdate on 05/18
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Overview

The online protection column is a chromatographic accessory installed between the injector and the analytical column in high-performance liquid chromatography, which passes through the mobile phase and sample solution. The protective column has a protective effect on the chromatographic column by pre capturing substances that can be firmly adsorbed by the analytical column but cannot be washed by the mobile phase, effectively extending the service life of the chromatographic column.

Product Details

Online protection pillarThe protective effect on the chromatographic column is achieved through the following two methods. Firstly, there is one at each end of the protective column coreaSieve plates, these two sieve plates have a certain filtering effect on the mobile phase, which can filter out particulate pollutants in the mobile phase and sample to prevent them from entering the chromatographic column and blocking the sieve plate and contaminating the column bed. Secondly, whenprotective columnWhen the packing material of the protective column is the same as that of the chromatographic column, the protective column can remove compounds that cause strong or irreversible adsorption with the packing material, which is also the reason why the packing material used for the protective column should be the same as that of the chromatographic column. By using these two methods, protecting the column can greatly improve the service life of the chromatographic column.

How to choose protection

1. Protect the filling material of the column and keep it consistent with the analysis column! Consistency in filler brand is essential to ensure consistent selectivity.

2. The specifications of the protective column should match those of the analytical column! The packing particle size is consistent, and the inner diameter of the protective column is similar to that of the analytical column.

3. For fine separation, the loading efficiency of the protective column itself should be good enough!

For fine separation or small inner diameter chromatographic column separation, the dead volume of the protective column connection should be as small as possible.

If it is used for UHPLC columns, the pressure resistance of the protective column should also be considered.

MorphlingTMOnline protection pillarThe replacement of the column core is convenient and fast, and it is easy to connect with the chromatographic column. It adopts a hand tight column sleeve, which can be tightened slightly with a wrench to achieve good sealing effect, and can withstand up to340 bar pressure. The filling material for protecting the column core and our company's MorphlingTMThe packing of the chromatographic column is the same, which can provide better protection for the chromatographic column. Different chromatographic columns can choose column cores bonded with different fillers.

Stainless steel protective columnMain technical parameters:

Stainless steel protective column

Imported fillersC18,C8,

Name

Specifications

protective column

Including 1 protective column cover, 1 column core, and 1 set of connectors

Replaceable column core

4.6×28mm