Peptide de novo sequencing - Biotec Biotech

Peptide de novo sequencing - Biotec BiotechIntroduction to Testing Technology Services Details

The de novo peptide sequencing technology plays a crucial role in the field of d à nb á izh ì omics research. This technology enables researchers to directly analyze the amino acid sequence of peptides or d à nb á izh ì without a reference sequence. The birth and development of de novo sequencing of peptides have significantly enhanced our understanding of the diversity and complexity of d à nb á izh ì, especially in analyzing unknown d à nb á izh ì, mutated d à nb á izh ì, or non model organisms, demonstrating its advantages.

1. Basic principles

Peptide de novo sequencing technology mainly relies on the combination of mass spectrometry technology and computational algorithms. The core of this technology lies in using mass spectrometry to perform high-precision mass analysis of peptide segments. Through techniques such as high-energy collision dissociation (HCD), collision induced dissociation (CID), and electron transfer dissociation (ETD), characteristic peptide fragment ions are generated. The mass to charge ratio (m/z value) of these fragment ions carries structural information of peptide amino acid residues. Subsequently, the computational algorithm analyzed these mass spectrometry data with the aim of reconstructing the amino acid sequence of the peptide segment. At present, commonly used calculation methods include spectrum matching algorithm, probability model algorithm, and neural network algorithm.

2. Technical process

The peptide de novo sequencing technology process mainly includes the following steps:

1. Sample preparation: Purification and desalination of peptide samples are carried out to ensure the release and purification of peptide segments, in order to reduce background interference and improve the sensitivity of mass spectrometry analysis.

2. Mass spectrometry analysis: Introduce the peptide mixture into a mass spectrometer for high-resolution mass spectrometry analysis. Currently, commonly used mass spectrometry instruments include quadrupole time-of-flight mass spectrometry (Q-TOF), linear ion trap mass spectrometry (LTQ), and orbital ion trap mass spectrometry (Orbitrap). The mass spectrometer will generate a large number of mass spectra, each containing peaks with different m/z values, which represent fragment ions of peptide segments.

3. Data analysis: Using Zhu ā ny è software tools, perform in-depth analysis of mass spectrometry data in order to reconstruct the amino acid sequence of peptide segments. This process requires comprehensive consideration of multiple factors, including the strength of fragment ions, the j ī ngqu è degree of m/z values, and the physicochemical properties of amino acids.

3. Application Fields

Peptide de novo sequencing technology has demonstrated its broad application potential in many fields:

In the field of proteomics research, this technology can assist researchers in identifying new peptides in the absence of reference sequences, thereby further expanding the omics database.

2. In the discovery of disease biomarkers, de novo sequencing technology can reveal novel peptide biomarkers associated with specific diseases, providing key clues for early diagnosis and treatment of diseases.

In the field of microbiology research, de novo sequencing technology can reveal the peptide composition of microorganisms in non model organisms or environmental samples, which helps to deepen our understanding of the ecological functions and evolutionary relationships of microorganisms.

4. In the process of drug development, de novo sequencing technology can be used to identify target proteins of drug action, optimize drug design and screening strategies.

Peptide de novo sequencing technology, as a key tool for d à nb á izh ì omics research, has demonstrated significant application potential in many fields.

Baitai Paike Biotechnology Characteristic Project

1、 Protein sequencing

Biotec Biotech uses Thermo's newly launched Obitrap Fusion Lumos mass spectrometer and Shimadzu's Edman degradation sequencing system to analyze the d à nb á izh ì sequence, providing mass spectrometry based protein sequencing analysis services, including amino acid composition analysis of d à nb á izh ì, Ndu ā nc è x ù, Cdu ā nc è x ù, and full sequence analysis, as well as d à nb á izh ì N-terminal sequence analysis services based on Edman degradation. For unknown theoretical sequences, we provide de novo sequencing services based on de novo sequencing to analyze protein sequences.

※ Service advantages:

1. Adopting the world's advanced mass spectrometer Obitrap Fusion Lumos;

2. It can achieve 100% coverage of the measured target protein sequence;

3. It can determine up to 70 amino acid sequences at the N-terminus of proteins;

4. Can measure various forms of samples: protein solution, PVDF protein bands;

5. Low sample usage: Protein samples only require 5-10ug to complete detection;

6. Sequencing is not affected by N-terminal modifications such as blocking, PEC, and glycosylation.

2、 D à nb á izh ì omics

Biotec Biotech uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with Nano LC to provide a variety of d à nb á izh ì omics analysis services, including quantitative d à nb á izh ì omics, targeted d à nb á izh ì omics, proteomics, post-translational modified proteomics, and more. In addition, Baitai Paike Biotechnology has launched a 4DD à nb á izh ì omics service based on timsTOF Pro, which helps with research in micro sample proteomics, large sample population medicine, and high-throughput modification genomics.

※ Service advantages:

1 . High throughput quantitative protein analysis: Large scale experimental analysis with multiple control groups, discovering new biomarkers;

2. Multiple in vitro and in vivo labeling methods are suitable for analyzing various samples such as tissues, cells, and blood;

3. High sensitivity of mass spectrometry analysis and high repeatability of experimental results;

4. Can detect low abundance proteins with a wide linear range;

5. Bioinformatics analysis is more systematic and accurate.

3、 Single cell mass spectrometry flow cytometry analysis

Baitai Paike Biotechnology uses the Fluidigm mass spectrometry flow cytometry system for single-cell mass spectrometry analysis. Metal element markers (usually metal element labeled specific antibodies) are used to label molecules on the surface and inside of cells. Then, individual cells are separated using flow cytometry principles, and the atomic mass spectrum of individual cells is analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The atomic mass spectrum data is then converted into signal molecule expression levels on the cell surface and inside.

※ Service advantages:

1. Advanced technology, filling technological gaps

The use of metal labeled antibody technology avoids the problem of few and easily interacting channels in traditional flow cytometry fluorescence. At the single-cell level, multiple indicators can be characterized simultaneously, and Baitai Paike Biotechnology can detect 51 target proteins simultaneously.

2. Large analysis quantity and low cost

Single cell RNAseq is limited by factors such as cost, and the total number of analyzed cells for all samples is generally around 2x10 ^ 4. However, flow cytometry technology can analyze at least 10 ^ 5 cells in one sample, achieving an order of magnitude increase, and the cost is not higher than that of single-cell RNAseq.

3. Great application prospects

① The results of flow cytometry can provide changes in cell subpopulations, which have great research prospects in clinical diagnosis, disease mechanism research, and other fields;

② Combining metal tag technology with other technologies will lead to new application directions. In addition to conventional proteins, mass spectrometry flow cytometry technology can also be used for post-translational modification of proteins;

③ Can detect cell survival rate, cell size, mRNA transcript expression level, DNA synthesis rate, and protease activity.

4、 Immunopeptide omics analysis and novel antigen discovery based on high-precision mass spectrometry

The one-stop solution for high-precision mass spectrometry based immunopeptidomic analysis and new antigen discovery from Biotec Biotech includes our proprietary, highly sensitive immunopeptide enrichment and identification scheme. We can assist you in identifying and recognizing over 10000 type I peptides and over 10000 type II peptides. Through our optimized high-throughput immunopeptidomic analysis platform, immunopeptidomic analysis can be performed with reproducible identification and quantification from small sample materials. This service can be applied to large-scale research, aiming to assist researchers in finding solutions for cancer, immune diseases, and infectious diseases, and to explore unknown targets in depth.

5、 Characterization of Biopharmaceuticals

Baitai Parker is based on high-resolution mass spectrometry technology, MALDI TOF， High performance chromatography separation technology provides a range of biological drug analysis solutions, from amino acid composition and primary structure analysis of biological products such as d à nb á izh ì, peptides, antibodies, vaccines, to product variability and purity analysis. Intended to provide high-quality biopharmaceutical analysis services and assist biopharmaceutical manufacturers in improving the quality of biopharmaceuticals.

Baitai Paike Biotechnology's Seven Major Testing Platforms

Baitai Paike Biotechnology - High quality service provider for characterization of biological products and multi omics of biological mass spectrometry

Beijing Baitai Paike Biotechnology Co., Ltd. is committed to providing quality control testing and project validation services for the biological/pharmaceutical and medical device industries. The company's laboratory follows regulations and guidelines such as NMPA, ICH, FDA, and EMA, and has obtained CNAS/ISO9001 dual quality system certification. It has established a complete quality system, including data cold and hot/remote backup, regular equipment measurement/periodic verification, software audit tracking, and provides customers with integrated solutions and technical services to support new drug research and development, drug application registration, and production release.

1. The company adopts the ISO9001 quality control system and provides CRO detection and analysis services based on mass spectrometry;

2. Recognized by the National CNAS Laboratory, we provide customers with drug quality research services that comply with global pharmaceutical regulations;

3. The business scope covers omics, proteomics, metabolomics, biopharmaceutical characterization, single-cell analysis, single-cell mass spectrometry flow cytometry, bioinformatics cloud analysis, and multi omics integrated analysis of biological mass spectrometry;

4. Seven major quality control testing platforms to meet your one-stop service needs;

5. Providing services to over 3000 enterprises and 10000+customers;

6. Committed to providing you with high-quality biological mass spectrometry analysis services!

Peptide de novo sequencing - Biotec BiotechIntroduction to Testing Technology Services Details