Parasitic egg modified Kato plate

Parasitic egg modified Kato plate

Introduction: The Improved Kato Method is a technique developed by the World Health Organization(WHO)*The method for detecting parasite eggs can simultaneously detect roundworm eggs, hookworm eggs, liver fluke eggs, and so on. This detection kit is used as an auxiliary tool for microscopic detection of parasite eggs in feces, which can improve the effectiveness of parasite detection and quantitatively analyze the degree of infection.

Specifications100 T/BoxApplicable test samples:

consumablescomposition

1Composite dye solution1bottle 50milliliter/bottle

2Quantitative board 100piece

3Scraper blade 100piece

4Nylon mesh 100Zhang

5Glass paper 100Zhang

6, glass slides 100piece

Operation steps:

1Place a nylon swab on the fecal sample to be tested, gently scrape it with a plastic scraper to allow fine fecal residue to pass through the nylon swab holes, and collect the filtered feces.

2Place the quantitative plate in the middle of the glass slide, and then fill the fine fecal residue on the scraper into the circular hole of the quantitative plate, filling the entire hole and smoothing it out carefullyRemove the quantitative plate and leave the fecal sample on the slide.

3、 取1 Soak the soaked hydrophilic glass paper, shake off excess transparent liquid, cover it on the fecal sample, and gently press it with a thick glass slide to evenly spread the fecal matter under the glass paper.

4Place the prepared Kato tablets in the specimen box (lined with absorbent paper)）.

5Room temperature after numbering25℃, humidity75%Time Schedule1 Hour or30~36After about half an hour in a temperature chamber, conduct a microscopic inspection. Microscopic examination and result judgment:

1Microscopic examination: Place the transparent Kato film on the stage of an optical microscope and examine it under a low magnification mirror（10×）Inspect under the microscope. Carefully examine every aspect through microscopic examination

Wild, and pay special attention to the identification of unfertilized roundworm eggs. Count the entire insect eggs (counting principle: count up but not down, count left but not right).

2Egg count of Kato tablets: based on the number of eggs detected by microscopic examination per tablet24 multiple(One stool, one test)），That is, the number of eggs per gram of feces（EPG）.calculate3 The number of schistosomiasis eggs in Zhang Kato's slice isATheBTheC, number of eggs per gram of feces(EPG)For:(A+B+C) ×8(One stool and three tests).

Notes:

1. Each fecal sample requires three smears (one stool and three tests to improve detection rate)

2. Cut glass paper should be twisted into pieces and placed in transparent liquid. Avoid putting it in a whole stack to prevent the inside from being soaked and affecting the transparency effect; SoakingTime cannot be less than24Hour.

3. The transparency time of thin shell eggs, such as hookworm eggs, cannot exceed2 Hour by hour, to avoid missed detections due to excessive transparency.

4. When the feces are hard, soak them in a little water or clear liquid for a moment, and then scrape them off.

5. The quantitative plate must be placed in the middle of the glass slide. If the quantitative plate is biased to one side, the fecal film at the edge of the slide cannot enter the microscopic field of view after slide preparationInternal leakage may occur.

6. When making specimens, excessive drying should be avoided to prevent deformation or rupture of the insect eggs, which may affect their identification.

7. The speed at which fecal residue becomes transparent depends on temperature, humidity, and the thickness of the fecal film. Generally, it can be left at room temperature, but in winter, it needs to be placed in a temperature chamber to accelerate transparency. LikeIf the fecal membrane is too thick, it is difficult to find the eggs.

This testing kit is stored at room temperature and contains a composite dye solution24 Valid within months, other materials * are valid.

Parasitic egg modified Kato plate