PAN fetal bovine serum ST30-3302

PANFetal Bovine SerumST30-3302

Brand:PAN

Item No.ST30-3302

Specifications500ml

Suzhou Qianshe Biotechnology has been focusing on biological reagents for many years, PAN ST30-3302, ST30-2602, ST30-3302P... More product numbers are available for sale and in stock supply!

Brand Introduction:

GermanyPAN-Biotech GmbHEstablished in1988Year, headquartered in Edenbach, Bavaria, Germany（Adenbach）, is a biotechnology enterprise specializing in cell culture related products.

PAN-BiotechMainly producing serum, culture medium, and cell culture related reagents;PAN SystechMainly engaged in the production and manufacturing of automated cell culture instruments.

Like other German brands, it has high-quality products made in Germany, and its entire line of products holds a considerable share in developed countries in Europe and America.

PANAll products produced by the company arecGMPProduced under production conditions, the product has been approved by the European Medicines Agency（EDQM）Certified and hasISO13485：2012andISO9001：2008Quality certification.

GermanyPANThe company's premium fetal bovine serum is a premium series selected through strict process screening, with strict quality control, small inter batch differences, good quality stability, low endotoxin content, no virus or mycoplasma, and high safety. Cell culture tests have shown that multiple cell lines exhibit better growth characteristics and are suitable for a wide variety of cell cultures.
GermanyPANDetailed description of fetal bovine serum:
1）Very low endotoxin content<1UI/mlInternational regulations: Premium fetal bovine serum<10 UI/ml）Extremely beneficial for cell growth and passage
2）Having the European Medicines Agency（EDQM）The product certificate issued complies with the European Pharmacopoeia1483Regulations
3）GMPProduced under conditions with a quality certification system certificate
4）Each batch of serum has a complete test report
5）Three times0.01 μmAseptic filtration treatment
6）Conduct bacterial, mycoplasma, virus, and virus antibody testing in accordance with the requirements of the International Animal Federation
7）Can be used according to customer requirementsγRadiation exposure

In the field of cell culture, many researchers struggle with a question when using serum:Should it be heat inactivated or not?

especially forFetal bovine serum（FBS）This is called“The golden nutrient solution for cells”Some laboratories follow old habits and habitually use them56℃heat treatment30minute, in order to“Inactivated complement”.

But today, we want to talk to everyone aboutFrom the perspective of developmental immunologyLet's clarify a conclusion:

For high-qualityFetal Bovine SerumMost experimentsNo longer requiring thermal inactivation treatment！

01What is the complement system?

The complement system is present in both humans and animalsInnate immune systemAn important component of, consisting of30Multiple plasma protein compositions. These proteins can:

·Directly damaging the cell membrane and inducing dissolution;

·Attract immune cells and amplify inflammatory responses;

·Cooperate with antibodies to clear antigens.

However, this complement“Attack Power”, inCell culture systemIt is often ainterfering factorEspecially forImmune cells or stem cellsThey are very sensitive to complement, ranging from poor condition to direct death in severe cases.

02Complement development in fetal cattle

Many people don't know,Fetal cow（fetal bovine）Not an immune mature individual.

From the perspective of embryonic development:

let me put it another way:The complement in fetal bovine serum is naturally low and has weak activity, lacking true cell killing ability.

03Heat inactivation may actually have the opposite effect?

The traditional heat inactivation operation isin56°Cwater bath30minuteUsed to destroy complement proteins.

But this step also has side effects:

❌degradedValuable growth factors and cytokines；

❌Increase protein aggregation and form precipitates;

❌The increase in inter batch differences affects the reproducibility of the experiment;

❌Shorten the shelf life and activity window of serum.

For modern technology preparationFBSproductDoing heat inactivation again is actually a method“pass

Why was cell culture used in the pastDo you want heat inactivated serum?

2010Before the year, the serum used for cell culture in China was mainly newborn bovine serum, with very little fetal bovine serum. With the large-scale slaughter of beef cattle, fetal bovine serum raw materials began to appear, gradually replacing newborn bovine serum. Therefore, in the era of newborn bovine serum, heat inactivation has a certain significance, and many laboratories have also developed a habit.

05What are the exceptions? Can still be thermally inactivated!

Although not necessary in most cases, in the following special situations,Heat inactivation still makes sense：

●Conduct complement dependent cytotoxicity experiments（CDC）The control group;

●Cultivate primary cells that are highly sensitive to complement, such asPBMC；

●In some animal experiments, to exclude complement variables;

●It is known that a certain batch of serum contains trace residual complement activity (determined by detection or pre-test).

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