PAN fetal bovine serum ST30-2602

PANFetal Bovine SerumST30-2602

Brand:PAN

Item No.ST30-2602ESGrade, suitable for stem cell culture

Specifications500ml

How to choose fetal bovine serum
one ⃣ ️Clearly identify the source:Nowadays, many brands promote products selected from Australia, New Zealand, and other places. It is important to check the origin of the packaging box and whether it matches the sales description to avoid changing the concept. Everyone can be atISIAFind detailed source information for serum on the website.
two ⃣ ️Routine testing indicators for fetal bovine serum: fetal bovine serumCOAIn China, conventional testing standards include endotoxins, total protein, mycoplasma, bacteria, viruses, osmotic pressure, total protein, etc. Certificate of Analysis for Fetal Bovine Serum Products（COAexactly.FBSAn important manifestation of quality control.

three ⃣ ️Appearance of fetal bovine serum: It appears light brown or amber in color. High quality fetal bovine serum should be a light yellow clear, non hemolytic, and slightly viscous liquid without foreign objects. However, the same serum from different batches may also have some color differences. If precipitation is found during purchase, it is usually the active substance in the serum, but it is also necessary to confirm with the salesperson whether there is a quality problem with the product.

four ⃣ ️How to store fetal bovine serum: when unopened-80After preservation and packaging, it can be determined according to usage, for example, if there are a large number of students in the sect, it can be-20preservation; If there are fewer members in the sect, after packaging-80preservation.4Save for no more than one month.

five ⃣ ️How to avoid contamination after packaging fetal bovine serum: Before packaging, be sure to50mlCentrifugal tubes exposed to ultraviolet radiation on ultra clean tables and biosafety cabinets30minAbove that, alcohol must be sprayed before entering the table with both hands during the packaging process. After packaging is completed, the lid should be promptly closed and sealed with a sealing film soaked in alcohol. transfer to-80/-20When in the refrigerator, prepare a clean plastic bag and store it uniformly after UV irradiation to avoid scattered placement.

Brand Introduction:

GermanyPAN-Biotech GmbHEstablished in1988Year, headquartered in Edenbach, Bavaria, Germany（Adenbach）, is a biotechnology enterprise specializing in cell culture related products.

PAN-BiotechMainly producing serum, culture medium, and cell culture related reagents;PAN SystechMainly engaged in the production and manufacturing of automated cell culture instruments.

Like other German brands, it has high-quality products made in Germany, and its entire line of products holds a considerable share in developed countries in Europe and America.

PANAll products produced by the company arecGMPProduced under production conditions, the product has been approved by the European Medicines Agency（EDQM）Certified and hasISO13485：2012andISO9001：2008Quality certification.

GermanyPANThe company's premium fetal bovine serum is a premium series selected through strict process screening, with strict quality control, small inter batch differences, good quality stability, low endotoxin content, no virus or mycoplasma, and high safety. Cell culture tests have shown that multiple cell lines exhibit better growth characteristics and are suitable for a wide variety of cell cultures.
GermanyPANDetailed description of fetal bovine serum:
1）Very low endotoxin content<1UI/mlInternational regulations: Premium fetal bovine serum<10 UI/ml）Extremely beneficial for cell growth and passage
2）Having the European Medicines Agency（EDQM）The product certificate issued complies with the European Pharmacopoeia1483Regulations
3）GMPProduced under conditions with a quality certification system certificate
4）Each batch of serum has a complete test report
5）Three times0.01 μmAseptic filtration treatment
6）Conduct bacterial, mycoplasma, virus, and virus antibody testing in accordance with the requirements of the International Animal Federation
7）Can be used according to customer requirementsγRadiation exposure

Key guidelines for the import and use of fetal bovine serum

1 Selection and identification of imported fetal bovine serum

There are often biotech companies in the market claiming that their fetal bovine serum is“Direct import from abroad”Or possess“Own blood collection base”Consumers need to carefully distinguish and master the following common sense:

1. Permitted import locations: According to China's agricultural regulations, in order to prevent the spread of diseases, only fetal bovine serum is allowed to be imported from specific agricultural and animal husbandry countries. At present, the main legal source countries are New Zealand, Australia, Uruguay, and Brazil. Serum from areas affected by mad cow disease or countries where foot-and-mouth disease has not been completely eradicated is prohibited from being imported.

2. Quality and origin correlation:

·Serum samples from Australia, New Zealand, and Uruguay are generally considered high-quality representatives. The reason is that it is mostly an island country or surrounded by water on three sides, with strict management of animal husbandry and extremely low risk of disease pollution.

·Brazilian serum has only been allowed to enter the Chinese market in recent years, and its quality is similar to Uruguayan serum.

3. Important identification point: It is necessary to verify the country of origin of the serum. If the certificate of origin presented by the company comes from a country other than the above-mentioned four countries, the product is considered an illegal import and cannot be purchased or used.

II Correct storage and use of fetal bovine serum

1. Long term storage: should be stored in-20°COr freeze and store at a lower temperature.

2. Correct thawing (recommended three-step method):

·Placed at room temperature for approximately1.5Hour.

·move to20°CWater bath approximately25minute

·Move again37°CWater bath approximately35Wait for minutes until Wan is completely thawed.

·Alternative solution: can be placed in advance4°CThaw the refrigerator overnight.

3. Post thaw handling and use:

·After thawing, please gently shake and mix well.

·Suggest small volume packaging to avoid repeated freeze-thaw cycles.

·If partial thawing occurs for some reason, it can be done before2-8°CAfter fully thawing and mixing, refreeze in-20°C.

·After preparing the serum containing Wan whole culture medium, it should be used as soon as possible, and it is recommended to use it up within one week.

·Avoid using serum or serum containing culture media37°CLet it sit for a long time to prevent nutrient degradation.

4. After thawing, store: thawed and unopened serum in a clean environment2-8°CIn the environment, up to a maximum of can be saved3About a week later, it will gradually become ineffective.

III About thermal inactivation

1. Function: Heat inactivation (usually56°Cwater bath30Minute is mainly used to inactivate the complement system in serum to prevent it from interfering with the results in certain specific experiments (such as smooth muscle contraction, immune cell activation, etc.).

2. Operation points:

·The serum must be completely thawed and mixed before proceeding.

·During the heating process, it is necessary to gently shake it regularly to ensure even heating.

·If heat inactivation is required after packaging, it is essential to ensure aseptic operation.

3. Is it necessary?

·In the vast majority of cases, it is not necessary. Research has shown that for most cell cultures, the promotion effect of correctly heat inactivated serum on cell growth is minimal, and may even inhibit cell growth due to heat treatment damaging growth factors or nutrients in the serum.

·Main disadvantage: Heat treatment significantly increases precipitation (visible under a microscope)“black dot”）The formation is easily mistaken for microbial contamination.

·Suggestion: Perform thermal inactivation only when explicitly required by the experimental protocol. For conventional cell culture, heat treatment is not recommended.

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