Oxoid trypsin powder bottle 500g

Oxoid trypsin powder bottle 500g

In the fields of microbiology experiments, fermentation engineering, and biopharmaceutical research and development, the nutrient ratio of the culture medium is one of the core variables that determine the growth of bacterial cells and the production of metabolites. Oxoid trypsin powder bottle is a high-quality peptone product produced by trypsin hydrolysis of casein. With its evenly distributed amino acid composition, amino acid nitrogen content, and century old brand reputation, it provides traceable nitrogen source guarantee for various culture medium formulations from basic research to large-scale production. This product has good physical properties such as light color, easy solubility, transparency, and no precipitation. It adopts a specification system that combines bottled and barrel packaging, fully covering different scale tasks such as routine bacterial strain recovery in clinical laboratories, industrial fermentation tank trial production, and biopharmaceutical sterility verification.

Choosing the Oxoid trypsin powder bottle means selecting a classic nitrogen source material that has been globally validated for nearly a century for your microbial amplification system.

1、 Product core advantage: nitrogen source guarantee derived from classic formulas

The core value of Oxoid trypsin powder bottle lies in its trypsin hydrolysis process derived from high-quality casein. Casein is the main protein in milk and a rich source of amino acid nitrogen. Its hydrolysis products are rich in peptides and amino acids, especially tryptophan, which is a good substrate for preparing biochemical detection culture media and is commonly used for testing indole reactions. Meanwhile, due to its high content of amino acid nitrogen, it can provide sufficient nitrogen source for bacterial protein synthesis, meeting the nutritional needs of various groups from heterotrophic aerobic bacteria to anaerobic bacteria.

Core physical and chemical parameters:

Typical analysis values of technical indicators (% w/w)

Total Nitrogen 13.3%

AminoNitrogen 3.7%

Sodium chloride 0.4%

PH (2% solution) 7.3 ± 0.2

The total nitrogen content of this product is ≥ 10% (up to 12.70%), the amino nitrogen content is controlled within the range of 3.4-4%, the inter batch difference is small, and the performance between batches is consistent. This high consistency of composition makes it a reliable component of culture media used in metabolic or growth research, especially in applications that require high performance and uniform composition. Its tryptophan content is high, so it can be used in the culture medium for testing indole reactions.

Core specification system:

LP0042B: 500g standard bottle, meeting the daily preparation needs of routine laboratories

LP0042T: 5kg laboratory economical packaging, suitable for continuous batch experiments with medium dosage

LP0042K: 25kg large specification process drum packaging, providing unit cost optimization solutions for large-scale production scenarios such as industrial fermentation and drug quality control

The multi specification system comprehensively covers the needs of tasks of different scales, from routine bacterial strain recovery in clinical laboratories, industrial fermentation tank trial production, to sterile validation in biopharmaceuticals.

2、 Widely applied in multiple fields: the cornerstone of nitrogen sources from drug quality control to food monitoring

The application range of Oxoid trypsin powder bottle covers multiple life science and industrial application fields, and is highly favored due to its wide compatibility.

Drug quality control and sterility testing: This product complies with the clear requirements of the United States Pharmacopeia (USP), European Pharmacopeia (EP), and other standards for "casein trypsin hydrolysate", providing strict compliance materials for QC processes such as antibiotic sterility testing and interferon diphtheria toxin production. It can be used as a sterile testing medium to effectively detect "flat acid" or sulfide spoilage bacteria in canned food.

The classic pairing of molecular biology and LB medium: For molecular biology laboratories, LB medium is the fundamental support for bacterial culture. Oxoid trypsin powder bottle and yeast powder (LP0021) form the basis of LB culture medium, which can maintain high copy plasmid amplification and protein expression in Escherichia coli. The combination of classic LB medium LP0042 and yeast powder LP0021 is a classic specification that OXOID in the UK has a large inventory of.

Biopharmaceutical fermentation industry: This product is one of the commonly used culture medium components in the fermentation industry, widely used in the production of pharmaceutical products such as antibiotics, extracellular proteins, interferons, and diphtheria toxoids. At the same time, it also plays an important role in fermentation industries such as vitamins, amino acids, organic acids, enzyme preparations, xanthan gum, biochemical products, and microbiological research.

Food and Environmental Microbial Monitoring: In environmental monitoring and food safety laboratories, culture media are used to prepare microbial detection media for drinking water, wastewater, and surface water, as well as detection media for total microbial count and coliform bacteria in food samples. At the same time, it is also a commonly used raw material for the preparation of bacterial biochemical characteristic test media such as sterile test media and anaerobic bacterial culture media.

3、 Complete specification system and inter batch consistency guarantee

Batch consistency is an important guarantee for this product. The technical specifications of LP0042B have been validated by typical batch numbers. Batch number 1938556 has an amino nitrogen content of 3.60%, total nitrogen content of 12.70%, drying loss of 4.30%, ash content of 7.90%, and pH value of 7.4. This high batch consistency enables Oxoid trypsin powder bottles to be effectively used in continuous experiments or large-scale production systems that require long-term cross batch tracking, reducing inter batch variability between culture medium raw materials and improving the reproducibility of fermentation target product synthesis.

Usage and Storage Guide

To fully utilize the performance of Oxoid trypsin powder bottle, the following preparation and storage suggestions are provided for reference.

Preparation and dissolution: This product has good physical properties such as light color, easy solubility, transparency, and no precipitation. It is recommended to weigh it in a clean environment and dissolve it in distilled water at 37-50 ℃ using a glass rod or magnetic stirrer. A 10% aqueous solution will dissolve.

Classic LB medium preparation: Weigh 10g of tryptone, 5g of yeast powder, and 10g of sodium chloride in 1L of medium, stir and dissolve, sterilize at 121 ℃ for half an hour under high pressure, cool to room temperature in a sterile environment, add bacterial solution, and shake overnight at 37 ℃ for cultivation.

Storage standard: It is recommended to store in a sealed manner at room temperature, away from light and under dry conditions. Long term stored original packaging should not be equipped with excessive inventory to prevent moisture absorption from affecting the activity of ingredients. Opened products should be used as soon as possible, and the bottle mouth should be reinforced with sealing film and stored in a dry cabinet away from light.

Production Quality System and Industry Certification

As a microbial product brand, OXOID adheres to Good Manufacturing Practice (GMP) standards to ensure that products meet technical specifications and regulatory requirements. It has passed FDA certification, ISO9001 certification, and ISO9000:2000 certification. The production process of its casein trypsin hydrolysate is completed under controlled conditions, resulting in a light color, easy solubility, transparency, and no precipitation of the product. This rigorous quality management system makes Oxoid trypsin powder bottled a reliable choice for nitrogen source raw materials in pharmaceutical, food testing, and biological product process development scenarios that require strict compliance management.

4、 Selection Decision Reference

When configuring core nitrogen source raw materials, researchers can comprehensively evaluate from the following aspects:

Conventional molecular biology experiments: Conduct routine drug sensitivity tests, microbial identification, and small protein expression. We recommend LP0042B (500g), which can be used to prepare approximately 50-100L of culture medium and is suitable for routine rotation experiments

Pilot scale up and process optimization: For the adaptation domestication of vaccine cell matrix, optimization of bioreactor process, and the team required for pilot scale up, it is recommended to use the LP0042T (5kg) specification

Large scale industrial fermentation: Users responsible for antibiotic industrial production and continuous inter batch supervision, 25kg LP0042K can effectively ensure the inter batch stability of the culture medium components

Compliance verification: Before writing a paper, applying for production approval, or submitting drug regulatory filing materials, please be sure to verify the batch quality inspection certificate (CoA) and follow the current pharmacopoeia general rules regarding the nutritional strength verification of pancreatic enzyme digestion raw materials, which can help improve the compliance and traceability of the culture medium scheme

summary

Oxoid trypsin powder bottle provides a reproducible nitrogen source reference for microbial strain cultivation, sterility validation, and biopharmaceutical fermentation engineering, thanks to its high-quality casein derived protein, 13.3% high total nitrogen, 3.7% amino nitrogen, and other balanced nutritional components. Bottled LP0042B (500g), 5kg LP0042T, and 25kg industrial specification LP0042K cover the dosage gradient from laboratory basic research to industrial production. Selecting appropriate specifications based on the nutritional requirements and budget of specific strains, and following standardized aseptic preparation operations and moisture-proof storage processes, will help significantly reduce the quality variation of the culture medium between batches and improve the reproducibility of amplification and fermentation target product synthesis.

Oxoid trypsin powder bottle 500gDirectly shipped from Hangzhou Qiannuo Biological Warehouse nationwide.