Abend centrifuge 5425/5425R in stock from distributors

Abend centrifuge 5425/5425R in stock from distributors

Centrifuge is one of the most frequently used equipment in molecular biology, cell biology, and clinical laboratory testing. As the two core models of the Eppendorf 5425 series, the Abend centrifuges 5425 and 5425R provide researchers with a complete centrifugation solution from conventional room temperature sample processing to temperature sensitive sample separation, with a capacity of 24 well microcentrifuge tubes and a stable centrifugal force of up to 21300 × g. The maximum centrifugal force, rotational speed, and capacity parameters of the two are the same, but the difference lies in the "R" (Refrigerated) at the end of the 5425R model. The 5425R integrates a compressor refrigeration system, making it an ideal choice for temperature sensitive experiments such as RNA extraction, enzyme reactions, and protein purification, while the 5425 excels in routine operations such as nucleic acid purification and cell precipitation that do not require cooling.

Choosing the Abend centrifuge 5425 or 5425R means equipping your laboratory with a globally validated dual track standardization scheme for micro separation work.

1、 Temperature control function: the key difference between the two

Upon closer inspection of these two mini centrifuges, one can notice that their differences are very subtle: they have almost identical appearances, consistent rotors and fluxes, and even maintain the same maximum centrifugal force. However, the quietly appearing "R" in the 5425R model represents "Refrigerated", which is the decisive difference between the two.

The 5425R is equipped with an advanced compressor refrigeration system, with a temperature range covering -10 ° C to+40 ° C and a temperature control accuracy of ± 1 ° C. This temperature control capability makes it particularly suitable for temperature sensitive sample processing scenarios, such as RNA extraction experiments, where low temperature environments can effectively inhibit RNase activity and prevent RNA degradation; In protein purification and enzymatic reaction processes, stable low-temperature conditions help maintain the natural conformation of enzyme proteins, ensuring sample integrity and subsequent biological activity.

In contrast, 5425 only operates in conventional centrifugation mode and cannot actively control the sample temperature during high-speed separation. It is suitable for basic experiments such as cell separation and serum extraction that do not require temperature control, and relies on its simple and stable characteristics to meet the daily centrifugation needs of most molecular biology experiments.

Silent OptiBowl ® Design: Create a peaceful experimental environment

The operating noise of centrifuges is often an important factor affecting the quality of experimental environments. The Abend centrifuges 5425/5425R are equipped with innovative OptiBowl technology ® Precise cavity design to weaken resonance propagation during operation from both structural and material perspectives. The average noise level during short-term operation (within 12 minutes) is only 45dB (A), far lower than that of conventional centrifuges; Even if used continuously for a long time, the maximum noise level is controlled below 51dB (A) (5425) or 54dB (A) (5425R).

In clinical testing, stem cell culture, and molecular diagnosis operations that require frequent centrifugation, quiet operation can ensure that centrifugation personnel can concentrate for a long time, especially suitable for placement in open experimental areas or shared equipment spaces that are sensitive to noise such as cell rooms.

QuickLock quick lock with 6 rotor options: convenient guarantee of compatibility from switch cover to sample

In terms of operational convenience, the Abend centrifuge 5425/5425R comes standard with Eppendorf's QuickLock quick locking technology. Users do not need additional tools, simply press with one hand to close the rotor cover and hear a crisp snap sound, completing the locking process; After centrifugation, the machine cover automatically opens to prevent the sample from overheating and easily remove the sample. For teams dealing with high-throughput samples, the cumulative time savings of quick touch based operations are very significant in their daily minds.

In terms of compatibility, the Abend centrifuge 5425/5425R is equipped with six interchangeable rotors, covering all the requirements for microtubes, PCR tubes, and centrifuge columns in daily molecular biology:

24 × 1.5/2.0mL standard angle rotor: equipped with airtight FA-24x2 rotor, suitable for precipitation collection in conventional microcentrifuge tubes;

PCR tube horizontal rotor: can accommodate 12 eight tube or 96 single tube at once;

10 × 5.0mL angle rotor: meets the pre-treatment requirements of medium capacity samples;

36 × 0.5mL small centrifuge tube adaptation: suitable for high-throughput small volume samples and blood sample tubes and protein extraction pretreatment;

EppendorKit rotor (F-45-18-11-Kit): specially optimized for the MiniPrep centrifuge column kit, the unique extended edge design provides support for the opened centrifuge tube cover during centrifugation, effectively preventing the tube cover from breaking during the elution stage.

The above rotors have been certified for third-party anti biological contamination airtightness and are suitable for safe centrifugation of hazardous samples.

Intelligent software functionality: adjusted to a new level of flexibility and ease of use

The Abend centrifuge 5425/5425R is equipped with enhanced software features aimed at reducing manual tuning time in repetitive experiments. Users can choose adjustable acceleration and braking slopes from 1 to 10 levels based on the fragility of the sample, to protect vulnerable cells or sensitive sediment. The 5425 model is equipped with three shortcut program buttons, which allow saving of all centrifugation schemes including speed, centrifugal force, time, and acceleration/deceleration curves, and one click access to commonly used experimental parameters, reducing operational errors caused by repeated settings. A research team that needs to refer to the same centrifugation process and use the same set of parameters for a long time is particularly suitable for maintaining batch centrifugation consistency by relying on this function for a long time.

2、 The core application scenario of 5425R: Low temperature protection throughout the entire experimental process

The low-temperature capability of 5425R covers various key applications in molecular biology and protein research.

RNA extraction and reverse transcription experiments: During the phenol chloroform extraction process from tissue or cell lysate, the sample is easily exposed to exogenous RNase at room temperature and rapidly degraded. 5425R is stabilized at 2-4 ℃ through pre cooling chamber and continuous low-temperature centrifugation, protecting the integrity of RNA and subsequent reverse transcription efficiency.

Enzyme reaction system and protein engineering: Short centrifugation after enzyme-linked immunosorbent assay or conversion reaction, centrifugal force may cause local heating of enzyme proteins and non-specific activation. The steady-state temperature control capability of 5425R under high-speed setting conditions enables highly temperature sensitive enzymatic reactions to operate reliably at low temperatures.

Sensitive temperature control in column purification: During the centrifugal elution process of DNA/RNA adsorption column assay kit, centrifugal force integrated with low-temperature protection can reduce repeated temperature fluctuations in high flux steps and ensure the repeatability of quality control data.

Laboratory daily centrifugation and large-scale high-throughput use

The Abend centrifuges 5425 and 5425R can meet most conventional and high-throughput separation requirements. The conventional steps of column lysis centrifugation, ethanol precipitation collection, etc. in nucleic acid purification can be completed using any of the above models. In experiments such as system preparation and rapid settling of multiple PCR products, or micro cell collection and subcellular organelle separation, this centrifuge also demonstrates stability and reliability. Two models paired with a 10 × 5.0mL adapter and a porous PCR horizontal rotor can significantly increase the number of samples per centrifugation.

3、 Selection Decision Framework and Procurement Suggestions

In the procurement process, users can refer to the following decision dimensions when determining whether to use the Abend centrifuge 5425 or 5425R:

Recommendation of decision-making dimensions and core considerations for selection

Sample sensitivity RNA extraction, enzymatic reaction, protein purification 5425R; the low-temperature function of conventional nucleic acid DNA/plasmid purification 54255425R is the key guarantee for sensitive samples

Budget and space constraints: Limited funds/tight space 5425; Pursuing temperature control function and multi-purpose use, 5425R5425 has a lower price and smaller size; 5425R expandable application areas

Both microcentrifuge tubes and PCR tubes with 24 well centrifugation are suitable for flux requirements; Batch sample collection of 5mL tubes can be selected with both options and 5mL angular rotors. Both options can be equipped with 6 types of rotors, with the same expansion capability

Both daily high load operation and long-term operation have maintenance free brushless motors, but the difference is that when selecting 5425R for temperature control, there will be an additional annual inspection of the refrigeration system

User evaluation of existing sample types and future development plans in the laboratory: mainly targeting the situation of conventional DNA/RNA purification and limited budget space, 5425 is the initial choice with better cost-effectiveness. If the experiment involves RNA stability, protein activity maintenance, and temperature control in enzymatic conversion, the temperature control function of 5425R will add extra repeatability and robustness to the experimental results.

summary

The Abend centrifuge 5425 covers daily microcentrifuge experiments with its compact design, extremely low noise, maintenance free motor, and intuitive shortcut key program design; The Abend centrifuge 5425R inherits the above advantages and adds precise temperature control (-10 ° C to+40 ° C) and rapid pre cooling function, providing precise cold environment control guarantee for temperature sensitive core experiments. Based on your laboratory budget, current sample types, and future project extension directions, selecting the appropriate model and following standardized centrifugal balancing and daily rotor maintenance procedures will help your micro sample pretreatment process to be more efficient and data consistent.

Abend centrifuge 5425/5425R in stock from distributorsHangzhou Qiannuo Biological Warehouse offers nationwide direct delivery of spot goods.