Optical sectioning of fluorescence imaging using Zeiss wide field microscope

The Apotome 3 optical slice of Zeiss wide field microscope fluorescence imaging can automatically recognize the magnification of the objective lens, move the matching grid into the optical path, use structural illumination to project the grid structure onto the focal plane of the sample, eliminate stray light in the non focal plane of the sample, and then generate clearer and sharper optical slices through Zeiss' algorithm, allowing you to obtain excellent resolution and high contrast images.

Optical sectioning of fluorescence imaging using Zeiss wide field microscopeApotome 3

When performing fluorescence imaging on larger samples, non focal plane stray light often blurs the image, thereby reducing contrast and resolution. The all-new Zeiss structured illumination Apotome 3 optical slicing imaging component can be mounted on wide field microscopes such as open mode inverted fluorescence microscopes, research grade upright fluorescence microscopes, and large field macroscopic zoom microscopes.Optical sectioning of fluorescence imaging using Zeiss wide field microscopeApotome 3 can automatically recognize the magnification of the objective lens, move the matching grid into the optical path, use structural illumination to project the grid structure onto the focal plane of the sample, eliminate stray light in the non focal plane of the sample, and then generate clearer and sharper optical slices through Zeiss' algorithm, allowing you to obtain excellent resolution and high contrast images. Compared with traditional wide field fluorescence microscopy images, Apotome 3 can significantly improve axial resolution, allowing you to obtain high-quality optical slices that support 3D rendering, including thick samples.

u High quality optical slicing: Zeiss Apotome3 features grids with three different geometric shapes, ensuring high resolution regardless of the magnification you choose,

u Free choice of light source and dye: Zeiss Apotome 3 can adapt to fluorescent groups and light sources. Therefore, when the complexity and requirements of the experiment change, you can also respond flexibly.

u More structured information: With the algorithm of structured illumination, you can even further improve image quality through deconvolution. Better identify the important structures of the inspected object.

u Two dimensional and three-dimensional fluorescence light cutting imaging of histological samples

u Two dimensional and three-dimensional fluorescence light cutting imaging of living cell samples

u Whole embryo large field fluorescence light sectioning imaging

Wide field image of cortical neuron DNA and microtubule staining (DAPI, A488), Z stack, 40X objective lens

(The left image was not captured using Apotome, while the right image was captured using Apotome)