New version of collagen detection kit BIOCOLOR agent

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

Product Category

Place of Origin

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Overview

Biocolor, a British company, launched a new version of its soluble collagen detection kit in September 2023. The samples that can be detected include: collagen content in the lungs, hearts, kidneys, skin, cartilage, intestines, fibroblasts, etc. of various species of mice, rats, humans, etc., with concentrations as low as 2 μ g/ml. 96 well plate model design, capable of detecting samples with serum.

Product Details

BIOCOLORSircol™ 2.0Soluble collagen protein detectiontest kit

BIOCOLORSircol™ 2.0Soluble collagen protein detectiontest kitProduct Overview

Sircol™ 2.0The soluble collagen detection kit is used for quantitative detection of soluble collagen from both in vivo and in vitro sources. This kit uses colorimetric detection and is based on96The microporous plate format enables precise and convenient in situ collagen analysis.

BIOCOLORSircol™ 2.0Application fields of soluble collagen protein detection kit

Measure soluble collagen in tissues such as muscles, lungs, liver, cartilage, tendons, and skin.
analysis2Dand3DSoluble collagen in cell culture.
Suitable for various sample types, including culture media, extracellular matrix, and other biological fluids.

BIOCOLORSircol™ 2.0Main features of soluble collagen protein detection kit

Enhanced specificity:By using an improved dye formula, the specificity of collagen has been enhanced10More than twice as much, far exceeding the interference of other proteins such as albumin.
Convenient format:The microplate format allows for the simultaneous processing of multiple samples, improving experimental consistency and reducing liquid transfer losses.
Wide sample compatibility:Can be used for various mammalian and non mammalian collagen sources, and can be adjusted as needed.

BIOCOLORSircol™ 2.0Soluble collagen detection kit, kit components

SircolDye reagent
Collagen reference standard
Plate washing reagent
Collagen Concentrating Reagent
neutralizing agent
Dye release reagent
96Hole detection microplate(1a)

BIOCOLORSircol™ 2.0Storage and stability of soluble collagen detection kit

Unopened: stored at room temperature, can be stably stored for one year.
After opening: Please store according to the specific storage guidelines provided in the manual to maintain the stability of the reagent.

BIOCOLORSircol™ 2.0Overview of the detection steps of soluble collagen protein detection kit

Sample preparation:Select an appropriate sample preparation plan based on the type and source of collagen.
Dye labeling:Translate into EnglishSircolAdd dye reagents to the sample in the microplate and shake it30minute
Centrifugal collection:Collect dye labeled collagen precipitates by centrifugation.
Cleaning and Release:Wash with dye release reagent and release the dye from the precipitate.
Absorption measurement:Using a microplate reader556 nmMeasure absorbance at wavelength.

BIOCOLORSircol™ 2.0Performance and sensitivity of soluble collagen protein detection kit

Detection range: 2 – 200 µg/mlWhen using collagen concentration scheme, it is0.25 – 20 µg/ml）.
Detection limit: 2 µg/ml(Standard Scheme) or0.25 µg/ml(Using a concentration scheme).

How to place an orderBIOCOLORSircol™ 2.0Soluble collagen detection kit

SIRC2 Sircol 2.0 SOLUBLE Collagen Assay (96 assays)