BIOCOLOR Cell Cycle Detection Kit Agent

BIOCOLOR C1000Cell-ClockOverview of Cell Cycle Detection Kit Products

Cell-ClockCell cycle detection kitIt is a detection system designed specifically for studying the stages of the living cell cycle. It provides clear color indications by detecting changes in mammalian cells at different stages of the cell cycle, enabling effective monitoringG1TheSTheG2andMThe distribution of cells during the period. This detection method does not require cell fixation or lysis, and can be directly labeled in live cells, making it suitable for various research applications such as cell proliferation and analysis of anti-cancer drug effects.

BIOCOLOR C1000Cell-ClockProduct features of cell cycle detection kit

• Real time monitoring of live cell cycleCan be used for the four main cell cycle stages in living cells（G1TheSTheG2andMTesting and analysis of the period.

• Quick and intuitive results: In1Staining and analysis will be completed within hours, and cells at different stages will be displayed in different colors for easy observation.

• Multiple application areasSuitable for research on cell cycle synchronization, anti-cancer drug screening, cell aging, etc.

• High sensitivity detectionThis kit can accurately quantify the proportion of cells at different stages of the cell cycle and assist image analysis software in precise data processing.

• Convenient and easy-to-use: Designed for12Kong or24The well cell culture plate can also adapt to other experimental formats.

BIOCOLOR C1000Cell-ClockApplication fields of cell cycle detection kit

• Cell proliferation researchUsed to monitor the cyclic changes of cells under different culture conditions, including the effects of medium composition, cell density, culture time, or cell passage times.

• Evaluation of anti-cancer drugsCan evaluate the effects and responses of known or potential anti-cancer drugs at a certain stage of the cell cycle, helping to study how drugs affect cell proliferation.

• Cell cycle synchronization experimentMonitor the synchronization of cell populations and their recovery time to the mixed cycle through the application of cell cycle arrest reagents.

• Cell aging and regenerationUsed to study the cyclic changes of cells with increasing age or passage times, especially in research related to cell regeneration and tissue repair.

BIOCOLOR C1000Cell-ClockCell cycle detection kit detection steps

1. cell culture: Spread the cells on24In the well culture plate, add to each well500 µlCulture medium, and in37°CCultivate to80%Above convergence.

2. Add experimental reagentsAfter removing the culture medium, add the experimental compound,37°CThe time required for incubation.

3. Dye labeling: Join150 µl Cell-ClockDye reagents, incubation60minute

4. Washing and photographyAfter dyeing, remove the dye and take a micrograph using an inverted microscope.

5. image analysis: UseImageJThe software performs pixel analysis of cell cycle stages to obtain the percentage of cells in each stage.

BIOCOLOR C1000Cell-ClockCell cycle detection kit, kit composition

• Cell-ClockDye reagentUsed for cell cycle stage labeling.

• tissue culture plate: Used for cell culture24Hole culture plate.

• Image analysis software: RequiredImageJImage processing software for data analysis (available for free).

SEOoptimize content

Why chooseCell-ClockCell cycle detection kit? Cell-ClockCell cycle detection kitIt is an ideal tool for studying cell cycle and cell proliferation. Through simple and rapid detection methods, researchers can easily monitor the distribution of cells at various stages of the cell cycle, which can be used for anti-cancer drug evaluation and cell cycle synchronization experiments. Its sensitivity and ease of operation make it a detection tool in cell biology research.

BIOCOLOR C1000Cell-ClockFrequently Asked Questions about Cell Cycle Detection Kit

Question:Cell-ClockWhat are the main advantages of cell cycle detection kits?
Answer: It can monitor the live cell cycle in real time without the need for cell fixation or lysis, and the staining time is only required1Hours, suitable for various experimental applications.

Q: Which experiments is this detection method applicable to?
Answer: It is suitable for research fields such as cell cycle analysis, anti-cancer drug effect research, cell aging and regeneration, and cell cycle synchronization.

Question: How to conduct data analysis?
Answer: UseImageJThe software can automatically analyze the pixels in the micrograph and obtainG1TheSTheG2andMThe proportion of phase cells.

BIOCOLOR C1000Cell-ClockOrdering Information for Cell Cycle Detection Kit

• Product Code：C1000（100Secondary testing) orC2000（400Secondary testing)

• Storage conditions：4°CStore away from light, stable when unopened12Months, after opening, it should be10Used up within days.