NCI-N87 human gastric cancer cells

1、 Overview of NCI-N87 Cell Products

NCI-N87 human gastric cancer cellsIt is a classic cell line widely used in biomedical research. This cell is derived from human gastric adenocarcinoma liver metastasis and has epithelial like growth characteristics. It proliferates in an adherent manner under in vitro culture conditions. The doubling time of NCI-N87 cells is about 30 hours, making it suitable for conducting various tumor biology research and drug screening experiments.

2、 NCI-N87 Cell Basic Information

3、 NCI-N87 Cell Characteristics and Biological Characteristics

NCI-N87 cells have distinct biological characteristics: MYC positive, erb-B2 positive, CEA and TAG72 expression. These unique molecular markers and biological behaviors make them important tools for researchers to conduct research on tumor mechanisms and drug development.

During the cultivation process, the cells exhibited stable proliferation ability and good experimental reproducibility, which can meet various in vitro experimental requirements. It is recommended to use STR identification to verify cell identity and ensure the reliability of experimental data.

4、 Cultivation conditions and operational suggestions

Culture medium configuration:RPMI-1640 + 10% FBS + 1% P/S。 The cultivation conditions are 37 ℃, 5% CO ₂, and saturated humidity. It is recommended to replace the fresh culture medium every 2-3 days, with a passage ratio of 1:2 to 1:4.

Cell revival:Quickly thaw the cryovial in a 37 ℃ water bath, centrifuge and resuspend in wan quan medium, and transfer to a culture bottle for cultivation.

Cell cryopreservation:Use a cryopreservation solution of 55% basic medium+40% FBS+5% DMSO and store in liquid nitrogen for a long time.

5、 Application Fields

NCI-N87 cells are widely used in the following research fields:

• Research on Targeted Therapy for Gastric Cancer: HER2 and Screening of Anti Gastric Cancer Drugs

• Sensitivity testing of anti-tumor drugs and optimization of treatment plans

• Screening and validation of tumor molecular markers

• Research on the Mechanism of Cell Signal Transduction Pathway

• Research on Tumor Microenvironment and Immune Escape

6、 Precautions

1. Cell culture must be carried out in a biosafety cabinet, strictly following sterile operating procedures.

The quality of culture medium and serum has a significant impact on cell growth, and it is recommended to use high-quality serum.

3. The cell state is affected by environmental factors such as temperature and pH, and regular observation of cell morphology and growth status is necessary.

4. This product is for scientific research purposes only and cannot be used for clinical diagnosis or treatment.

5. After receiving the cells, please conduct STR identification in a timely manner to confirm the identity of the cells.

7、 Product specifications and ordering information

If you need to learn more about NCI-N87 cell products or obtain technical support, please feel free to contact our professional customer service team. We will provide you with free guidance on cell culture technology throughout the process to ensure the smooth progress of your scientific research work.