SPF grade pathogen quality control multiplex qPCR detection kit for large and small mice (2 common virus detection items)

Multi qPCR detection kit for common viruses in SPF grade rats and mice

(Probe method)

SPF. M/R-CV-001 50 T/ 盒

Biowing^®QPCR detection kit for common viruses in SPF grade rats and mice，Multi qPCR detection kit for common viruses in SPF grade rats and mice，Designed for two viruses that are susceptible to experimental mice, mouse hepatitis virus and mouse norovirus.

This kit uses two sets of primer probesFAM (Mouse Hepatitis Virus MHV) and HEX (Mouse Norovirus MNV) channels are used to detect two viruses, and a set of primer probes is used to detect internal reference RNA in CY5 channel. This kit has the following characteristics:

sensitiveHigh detection sensitivity,10 copies/μ L can be effectively detected.

stableFull process closed pipe operation without cross contamination.

reliableContains internal reference control to avoid false negatives.

convenientEasy to operate, one-step detection.

Transportation and Storage Conditions

Low temperature freezing transportation,-Stored at 20 ℃, valid for 1 year; After use, it should still be stored at -20 ℃ and should not be repeatedly frozen or thawed more than three times.

A BOX:

B BOX:

Note:

Positive quality control（PC contains three types of viral nucleic acids or plasmid DNA containing specific fragments, namely mouse hepatitis virus MHV, mouse norovirus MNV, and internal reference RNA.

【 Precautions 】

Due toThe PCR reaction is very sensitive, and the following precautions should be taken during experimental operations to avoid nucleic acid contamination.

1. Suggest partitioning operation

（1) Zone A: (Clean area) Prepare Mix and qPCR negative samples, it is recommended to complete them on an ultra clean workbench, and provide clean equipment and sterile enzyme free consumables specifically for system preparation.

（2) Zone B: (Nucleic acid extraction and sample adding area) Sample DNA/RNA extraction is recommended to be completed on the ultra clean workbench. If there is no ultra clean workbench, please extract under ventilation and immediately disinfection and sterilization it. It needs to be equipped with equipment dedicated to nucleic acid extraction, and conduct disinfection and sterilization treatment on the environment and equipment after each extraction; In the sample addition area, samples should be added in the order of adding the test sample first and then the positive control. The arrangement of the positive control on the qPCR reaction plate should be away from the test sample well and the negative control; It is also necessary to provide a special instrument for adding samples, and conduct nucleic acid disinfection and sterilization after each addition.

（3) Area C: (Nucleic acid amplification area) This area is equipped with fluorescence quantitative PCR instrument and related computers for data processing, and also needs regular nucleic acid disinfection and sterilization.